In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.

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Autores principales:	Xiaoyu Chen, Josephine M. Janssen, Jin Liu, Ignazio Maggio, Anke E. J. ‘t Jong, Harald M.M. Mikkers, Manuel A. F. V. Gonçalves
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/60102009427545b88a37146eedf8137c
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spelling	oai:doaj.org-article:60102009427545b88a37146eedf8137c2021-12-02T14:42:09ZIn trans paired nicking triggers seamless genome editing without double-stranded DNA cutting10.1038/s41467-017-00687-12041-1723https://doaj.org/article/60102009427545b88a37146eedf8137c2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00687-1https://doaj.org/toc/2041-1723CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.Xiaoyu ChenJosephine M. JanssenJin LiuIgnazio MaggioAnke E. J. ‘t JongHarald M.M. MikkersManuel A. F. V. GonçalvesNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
description	CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.
format	article
author	Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves
author_facet	Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves
author_sort	Xiaoyu Chen
title	In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
title_short	In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
title_full	In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
title_fullStr	In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
title_full_unstemmed	In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
title_sort	in trans paired nicking triggers seamless genome editing without double-stranded dna cutting
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/60102009427545b88a37146eedf8137c
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In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

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