The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs.
In a search for secondary plant compounds that bind to the glucocorticoid receptor (GR), the cyclobutane lignan endiandrin A was discovered from the rainforest tree Endiandra anthropophagorum Domin. Our present study aims to characterize the effect of endiandrin A on GR-dependent induction of coloni...
Guardado en:
Autores principales: | , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2012
|
Materias: | |
Acceso en línea: | https://doaj.org/article/6195fe6796c545c2af4270a22eee55dc |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:6195fe6796c545c2af4270a22eee55dc |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:6195fe6796c545c2af4270a22eee55dc2021-11-18T08:08:59ZThe plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs.1932-620310.1371/journal.pone.0049426https://doaj.org/article/6195fe6796c545c2af4270a22eee55dc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23152905/?tool=EBIhttps://doaj.org/toc/1932-6203In a search for secondary plant compounds that bind to the glucocorticoid receptor (GR), the cyclobutane lignan endiandrin A was discovered from the rainforest tree Endiandra anthropophagorum Domin. Our present study aims to characterize the effect of endiandrin A on GR-dependent induction of colonic sodium transport. The effect of endiandrin A was analyzed in GR-expressing colonic HT-29/B6 cells (HT-29/B6-GR). GR transactivation and subcellular localization were investigated by reporter gene assay and immunofluorescence. Epithelial sodium channel (ENaC) was analyzed by qRT-PCR and by measuring amiloride-sensitive short-circuit current (I(sc)) in Ussing chambers. Endiandrin A (End A) has been identified as GR receptor binder. However, it did not cause significant GR transactivation as pGRE-luciferase activity was only 7% of that of the maximum effect of dexamethasone. Interestingly, endiandrin A had a significant impact on dexamethasone-dependent sodium absorption in cells co-exposed to tumor necrosis factor (TNF)-α. This was in part due to up-regulation of β- and γ-ENaC subunit expression. Endiandrin A potentiated GR-mediated transcription by increasing GR protein expression and phosphorylation. It inhibited c-Jun N-terminal kinase (JNK) activation induced by dexamethasone and/or TNF-α and increased levels of GR localized to the nucleus. Additionally, endiandrin A increased the serum- and glucocorticoid-induced kinase (sgk)-1 via activation of p38. Finally, the regulation of ENaC function by endiandrin A was confirmed in rat native colon. In conclusion, endiandrin A potentiates glucocorticoid-driven activation of colonic epithelial sodium channels via JNK inhibition and p38 activation due to transcriptional up-regulation of β- and γ-ENaC-subunits along with induction of sgk-1.Dana KuntzschTheresa BergannPetra DamesAnja FrommMichael FrommRohan A DavisMatthias F MelzigJoerg D SchulzkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49426 (2012) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Dana Kuntzsch Theresa Bergann Petra Dames Anja Fromm Michael Fromm Rohan A Davis Matthias F Melzig Joerg D Schulzke The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
description |
In a search for secondary plant compounds that bind to the glucocorticoid receptor (GR), the cyclobutane lignan endiandrin A was discovered from the rainforest tree Endiandra anthropophagorum Domin. Our present study aims to characterize the effect of endiandrin A on GR-dependent induction of colonic sodium transport. The effect of endiandrin A was analyzed in GR-expressing colonic HT-29/B6 cells (HT-29/B6-GR). GR transactivation and subcellular localization were investigated by reporter gene assay and immunofluorescence. Epithelial sodium channel (ENaC) was analyzed by qRT-PCR and by measuring amiloride-sensitive short-circuit current (I(sc)) in Ussing chambers. Endiandrin A (End A) has been identified as GR receptor binder. However, it did not cause significant GR transactivation as pGRE-luciferase activity was only 7% of that of the maximum effect of dexamethasone. Interestingly, endiandrin A had a significant impact on dexamethasone-dependent sodium absorption in cells co-exposed to tumor necrosis factor (TNF)-α. This was in part due to up-regulation of β- and γ-ENaC subunit expression. Endiandrin A potentiated GR-mediated transcription by increasing GR protein expression and phosphorylation. It inhibited c-Jun N-terminal kinase (JNK) activation induced by dexamethasone and/or TNF-α and increased levels of GR localized to the nucleus. Additionally, endiandrin A increased the serum- and glucocorticoid-induced kinase (sgk)-1 via activation of p38. Finally, the regulation of ENaC function by endiandrin A was confirmed in rat native colon. In conclusion, endiandrin A potentiates glucocorticoid-driven activation of colonic epithelial sodium channels via JNK inhibition and p38 activation due to transcriptional up-regulation of β- and γ-ENaC-subunits along with induction of sgk-1. |
format |
article |
author |
Dana Kuntzsch Theresa Bergann Petra Dames Anja Fromm Michael Fromm Rohan A Davis Matthias F Melzig Joerg D Schulzke |
author_facet |
Dana Kuntzsch Theresa Bergann Petra Dames Anja Fromm Michael Fromm Rohan A Davis Matthias F Melzig Joerg D Schulzke |
author_sort |
Dana Kuntzsch |
title |
The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
title_short |
The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
title_full |
The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
title_fullStr |
The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
title_full_unstemmed |
The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs. |
title_sort |
plant-derived glucocorticoid receptor agonist endiandrin a acts as co-stimulator of colonic epithelial sodium channels (enac) via sgk-1 and mapks. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doaj.org/article/6195fe6796c545c2af4270a22eee55dc |
work_keys_str_mv |
AT danakuntzsch theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT theresabergann theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT petradames theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT anjafromm theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT michaelfromm theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT rohanadavis theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT matthiasfmelzig theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT joergdschulzke theplantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT danakuntzsch plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT theresabergann plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT petradames plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT anjafromm plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT michaelfromm plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT rohanadavis plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT matthiasfmelzig plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks AT joergdschulzke plantderivedglucocorticoidreceptoragonistendiandrinaactsascostimulatorofcolonicepithelialsodiumchannelsenacviasgk1andmapks |
_version_ |
1718422170370048000 |