In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination

Abstract Novel tuberculosis (TB)-vaccines preferably should (i) boost host immune responses induced by previous BCG vaccination and (ii) be directed against Mycobacterium tuberculosis (Mtb) proteins expressed throughout the Mtb infection-cycle. Human Mtb antigen-discovery screens identified antigens...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariateresa Coppola, Fabienne Jurion, Susan J. F. van den Eeden, Hermann Giresse Tima, Kees L. M. C. Franken, Annemieke Geluk, Marta Romano, Tom H. M. Ottenhoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/61b3fb75b0394d3298244d9977413a15
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:61b3fb75b0394d3298244d9977413a15
record_format	dspace
spelling	oai:doaj.org-article:61b3fb75b0394d3298244d9977413a152021-12-02T17:50:41ZIn-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination10.1038/s41541-021-00343-22059-0105https://doaj.org/article/61b3fb75b0394d3298244d9977413a152021-06-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00343-2https://doaj.org/toc/2059-0105Abstract Novel tuberculosis (TB)-vaccines preferably should (i) boost host immune responses induced by previous BCG vaccination and (ii) be directed against Mycobacterium tuberculosis (Mtb) proteins expressed throughout the Mtb infection-cycle. Human Mtb antigen-discovery screens identified antigens encoded by Mtb-genes highly expressed during in vivo murine infection (IVE-TB antigens). To translate these findings towards animal models, we determined which IVE-TB-antigens are recognised by T-cells following Mtb challenge or BCG vaccination in three different mouse strains. Eleven Mtb-antigens were recognised across TB-resistant and susceptible mice. Confirming previous human data, several Mtb-antigens induced cytokines other than IFN-γ. Pulmonary cells from susceptible C3HeB/FeJ mice produced less TNF-α, agreeing with the TB-susceptibility phenotype. In addition, responses to several antigens were induced by BCG in C3HeB/FeJ mice, offering potential for boosting. Thus, recognition of promising Mtb-antigens identified in humans validates across multiple mouse TB-infection models with widely differing TB-susceptibilities. This offers translational tools to evaluate IVE-TB-antigens as diagnostic and vaccine antigens.Mariateresa CoppolaFabienne JurionSusan J. F. van den EedenHermann Giresse TimaKees L. M. C. FrankenAnnemieke GelukMarta RomanoTom H. M. OttenhoffNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mariateresa Coppola Fabienne Jurion Susan J. F. van den Eeden Hermann Giresse Tima Kees L. M. C. Franken Annemieke Geluk Marta Romano Tom H. M. Ottenhoff In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
description	Abstract Novel tuberculosis (TB)-vaccines preferably should (i) boost host immune responses induced by previous BCG vaccination and (ii) be directed against Mycobacterium tuberculosis (Mtb) proteins expressed throughout the Mtb infection-cycle. Human Mtb antigen-discovery screens identified antigens encoded by Mtb-genes highly expressed during in vivo murine infection (IVE-TB antigens). To translate these findings towards animal models, we determined which IVE-TB-antigens are recognised by T-cells following Mtb challenge or BCG vaccination in three different mouse strains. Eleven Mtb-antigens were recognised across TB-resistant and susceptible mice. Confirming previous human data, several Mtb-antigens induced cytokines other than IFN-γ. Pulmonary cells from susceptible C3HeB/FeJ mice produced less TNF-α, agreeing with the TB-susceptibility phenotype. In addition, responses to several antigens were induced by BCG in C3HeB/FeJ mice, offering potential for boosting. Thus, recognition of promising Mtb-antigens identified in humans validates across multiple mouse TB-infection models with widely differing TB-susceptibilities. This offers translational tools to evaluate IVE-TB-antigens as diagnostic and vaccine antigens.
format	article
author	Mariateresa Coppola Fabienne Jurion Susan J. F. van den Eeden Hermann Giresse Tima Kees L. M. C. Franken Annemieke Geluk Marta Romano Tom H. M. Ottenhoff
author_facet	Mariateresa Coppola Fabienne Jurion Susan J. F. van den Eeden Hermann Giresse Tima Kees L. M. C. Franken Annemieke Geluk Marta Romano Tom H. M. Ottenhoff
author_sort	Mariateresa Coppola
title	In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
title_short	In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
title_full	In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
title_fullStr	In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
title_full_unstemmed	In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination
title_sort	in-vivo expressed mycobacterium tuberculosis antigens recognised in three mouse strains after infection and bcg vaccination
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61b3fb75b0394d3298244d9977413a15
work_keys_str_mv	AT mariateresacoppola invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT fabiennejurion invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT susanjfvandeneeden invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT hermanngiressetima invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT keeslmcfranken invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT annemiekegeluk invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT martaromano invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination AT tomhmottenhoff invivoexpressedmycobacteriumtuberculosisantigensrecognisedinthreemousestrainsafterinfectionandbcgvaccination
_version_	1718379288403640320

In-vivo expressed Mycobacterium tuberculosis antigens recognised in three mouse strains after infection and BCG vaccination

Ejemplares similares