Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines

In an imaging-based screen of >3,300 compounds compounds, Soboleva et al identify HDAC inhibitors as mediators of erythroid cell enucleation. They further show that the erythroid-specific cell membrane protein, SPTA1, is downregulated in HDAC inhibited cells and that restoration of SPTA1 expressi...

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Autores principales:	Svetlana Soboleva, Ryo Kurita, Fredrik Ek, Hugo Åkerstrand, Rita Silvério-Alves, Roger Olsson, Yukio Nakamura, Kenichi Miharada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/61f572de14eb48a18df29210fe97c443
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spelling	oai:doaj.org-article:61f572de14eb48a18df29210fe97c4432021-12-02T15:02:48ZIdentification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines10.1038/s42003-021-02202-12399-3642https://doaj.org/article/61f572de14eb48a18df29210fe97c4432021-06-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02202-1https://doaj.org/toc/2399-3642In an imaging-based screen of >3,300 compounds compounds, Soboleva et al identify HDAC inhibitors as mediators of erythroid cell enucleation. They further show that the erythroid-specific cell membrane protein, SPTA1, is downregulated in HDAC inhibited cells and that restoration of SPTA1 expression using CRISPR-activation partially rescues the fragility of cells, improving enucleation efficiency.Svetlana SobolevaRyo KuritaFredrik EkHugo ÅkerstrandRita Silvério-AlvesRoger OlssonYukio NakamuraKenichi MiharadaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Svetlana Soboleva Ryo Kurita Fredrik Ek Hugo Åkerstrand Rita Silvério-Alves Roger Olsson Yukio Nakamura Kenichi Miharada Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
description	In an imaging-based screen of >3,300 compounds compounds, Soboleva et al identify HDAC inhibitors as mediators of erythroid cell enucleation. They further show that the erythroid-specific cell membrane protein, SPTA1, is downregulated in HDAC inhibited cells and that restoration of SPTA1 expression using CRISPR-activation partially rescues the fragility of cells, improving enucleation efficiency.
format	article
author	Svetlana Soboleva Ryo Kurita Fredrik Ek Hugo Åkerstrand Rita Silvério-Alves Roger Olsson Yukio Nakamura Kenichi Miharada
author_facet	Svetlana Soboleva Ryo Kurita Fredrik Ek Hugo Åkerstrand Rita Silvério-Alves Roger Olsson Yukio Nakamura Kenichi Miharada
author_sort	Svetlana Soboleva
title	Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
title_short	Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
title_full	Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
title_fullStr	Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
title_full_unstemmed	Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
title_sort	identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61f572de14eb48a18df29210fe97c443
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_version_	1718389080500207616

Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines

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