Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water

The H2S test was created to assess the microbial quality of drinking water in low-resource settings, but the original version of the H2S test lacks sensitivity and specificity for faecal indicator bacteria. There is evidence that a modified media formula of the H2S test may be more sensitive and spe...

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Autores principales:	Bastian Schnabel, Jonathan Lance Caplin, Ian Richard Cooper
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	drinking water quality faecal indicator bacteria h2s test method development sulphur-reducing bacteria water quality monitoring Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506
Acceso en línea:	https://doaj.org/article/6270b4b13d334b0ab9f8025abf1b7fa6
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spelling	oai:doaj.org-article:6270b4b13d334b0ab9f8025abf1b7fa62021-11-06T07:05:17ZModification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water1606-97491607-079810.2166/ws.2020.301https://doaj.org/article/6270b4b13d334b0ab9f8025abf1b7fa62021-02-01T00:00:00Zhttp://ws.iwaponline.com/content/21/1/59https://doaj.org/toc/1606-9749https://doaj.org/toc/1607-0798The H2S test was created to assess the microbial quality of drinking water in low-resource settings, but the original version of the H2S test lacks sensitivity and specificity for faecal indicator bacteria. There is evidence that a modified media formula of the H2S test may be more sensitive and specific for the faecal indicator bacterium Escherichia coli (E. coli) and less sensitive to organisms of non-faecal origin. This research established the detection threshold and operational range of the H2S test, to increase its sensitivity and specificity for E. coli. A total of 20 modifications of the H2S test, and the original test, were assayed against 20 confirmed and pure culture bacteria of faecal and non-faecal origin at varying concentrations. Additionally, some of the H2S test modifications were evaluated against standard methods for drinking-water analysis. Results indicate that using a modified version of the H2S test containing L-cystine and 2-mercaptopyridine, and bile salts or penicillin G, E. coli will produce H2S. In addition, this research reveals which organisms react positively to the original and modified versions of the H2S test. The modified versions of the H2S test can be promoted as a simple screening test for microbial drinking-water safety in low-resource settings.Bastian SchnabelJonathan Lance CaplinIan Richard CooperIWA Publishingarticledrinking water qualityfaecal indicator bacteriah2s testmethod developmentsulphur-reducing bacteriawater quality monitoringWater supply for domestic and industrial purposesTD201-500River, lake, and water-supply engineering (General)TC401-506ENWater Supply, Vol 21, Iss 1, Pp 59-79 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drinking water quality faecal indicator bacteria h2s test method development sulphur-reducing bacteria water quality monitoring Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506
spellingShingle	drinking water quality faecal indicator bacteria h2s test method development sulphur-reducing bacteria water quality monitoring Water supply for domestic and industrial purposes TD201-500 River, lake, and water-supply engineering (General) TC401-506 Bastian Schnabel Jonathan Lance Caplin Ian Richard Cooper Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
description	The H2S test was created to assess the microbial quality of drinking water in low-resource settings, but the original version of the H2S test lacks sensitivity and specificity for faecal indicator bacteria. There is evidence that a modified media formula of the H2S test may be more sensitive and specific for the faecal indicator bacterium Escherichia coli (E. coli) and less sensitive to organisms of non-faecal origin. This research established the detection threshold and operational range of the H2S test, to increase its sensitivity and specificity for E. coli. A total of 20 modifications of the H2S test, and the original test, were assayed against 20 confirmed and pure culture bacteria of faecal and non-faecal origin at varying concentrations. Additionally, some of the H2S test modifications were evaluated against standard methods for drinking-water analysis. Results indicate that using a modified version of the H2S test containing L-cystine and 2-mercaptopyridine, and bile salts or penicillin G, E. coli will produce H2S. In addition, this research reveals which organisms react positively to the original and modified versions of the H2S test. The modified versions of the H2S test can be promoted as a simple screening test for microbial drinking-water safety in low-resource settings.
format	article
author	Bastian Schnabel Jonathan Lance Caplin Ian Richard Cooper
author_facet	Bastian Schnabel Jonathan Lance Caplin Ian Richard Cooper
author_sort	Bastian Schnabel
title	Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
title_short	Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
title_full	Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
title_fullStr	Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
title_full_unstemmed	Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
title_sort	modification of the h2s test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/6270b4b13d334b0ab9f8025abf1b7fa6
work_keys_str_mv	AT bastianschnabel modificationoftheh2stesttoscreenforthedetectionofsulphurandsulphatereducingbacteriaoffaecalorigininwater AT jonathanlancecaplin modificationoftheh2stesttoscreenforthedetectionofsulphurandsulphatereducingbacteriaoffaecalorigininwater AT ianrichardcooper modificationoftheh2stesttoscreenforthedetectionofsulphurandsulphatereducingbacteriaoffaecalorigininwater
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Modification of the H2S test to screen for the detection of sulphur- and sulphate-reducing bacteria of faecal origin in water

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