Deep and clear optical imaging of thick inhomogeneous samples.

Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a...

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Autores principales:	Raphael Jorand, Gwénaële Le Corre, Jordi Andilla, Amina Maandhui, Céline Frongia, Valérie Lobjois, Bernard Ducommun, Corinne Lorenzo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/629f34a6720b44a7bc26b06e91918903
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id	oai:doaj.org-article:629f34a6720b44a7bc26b06e91918903
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spelling	oai:doaj.org-article:629f34a6720b44a7bc26b06e919189032021-11-18T07:20:55ZDeep and clear optical imaging of thick inhomogeneous samples.1932-620310.1371/journal.pone.0035795https://doaj.org/article/629f34a6720b44a7bc26b06e919189032012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558226/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.Raphael JorandGwénaële Le CorreJordi AndillaAmina MaandhuiCéline FrongiaValérie LobjoisBernard DucommunCorinne LorenzoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35795 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raphael Jorand Gwénaële Le Corre Jordi Andilla Amina Maandhui Céline Frongia Valérie Lobjois Bernard Ducommun Corinne Lorenzo Deep and clear optical imaging of thick inhomogeneous samples.
description	Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.
format	article
author	Raphael Jorand Gwénaële Le Corre Jordi Andilla Amina Maandhui Céline Frongia Valérie Lobjois Bernard Ducommun Corinne Lorenzo
author_facet	Raphael Jorand Gwénaële Le Corre Jordi Andilla Amina Maandhui Céline Frongia Valérie Lobjois Bernard Ducommun Corinne Lorenzo
author_sort	Raphael Jorand
title	Deep and clear optical imaging of thick inhomogeneous samples.
title_short	Deep and clear optical imaging of thick inhomogeneous samples.
title_full	Deep and clear optical imaging of thick inhomogeneous samples.
title_fullStr	Deep and clear optical imaging of thick inhomogeneous samples.
title_full_unstemmed	Deep and clear optical imaging of thick inhomogeneous samples.
title_sort	deep and clear optical imaging of thick inhomogeneous samples.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/629f34a6720b44a7bc26b06e91918903
work_keys_str_mv	AT raphaeljorand deepandclearopticalimagingofthickinhomogeneoussamples AT gwenaelelecorre deepandclearopticalimagingofthickinhomogeneoussamples AT jordiandilla deepandclearopticalimagingofthickinhomogeneoussamples AT aminamaandhui deepandclearopticalimagingofthickinhomogeneoussamples AT celinefrongia deepandclearopticalimagingofthickinhomogeneoussamples AT valerielobjois deepandclearopticalimagingofthickinhomogeneoussamples AT bernardducommun deepandclearopticalimagingofthickinhomogeneoussamples AT corinnelorenzo deepandclearopticalimagingofthickinhomogeneoussamples
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Deep and clear optical imaging of thick inhomogeneous samples.

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