Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen

Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.

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Auteurs principaux:	Daniel P. Depledge, Kalanghad Puthankalam Srinivas, Tomohiko Sadaoka, Devin Bready, Yasuko Mori, Dimitris G. Placantonakis, Ian Mohr, Angus C. Wilson
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/62e6f7e4a2564e61a8a33f67a99d8972
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spelling	oai:doaj.org-article:62e6f7e4a2564e61a8a33f67a99d89722021-12-02T16:58:18ZDirect RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen10.1038/s41467-019-08734-92041-1723https://doaj.org/article/62e6f7e4a2564e61a8a33f67a99d89722019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08734-9https://doaj.org/toc/2041-1723Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.Daniel P. DepledgeKalanghad Puthankalam SrinivasTomohiko SadaokaDevin BreadyYasuko MoriDimitris G. PlacantonakisIan MohrAngus C. WilsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Daniel P. Depledge Kalanghad Puthankalam Srinivas Tomohiko Sadaoka Devin Bready Yasuko Mori Dimitris G. Placantonakis Ian Mohr Angus C. Wilson Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
description	Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.
format	article
author	Daniel P. Depledge Kalanghad Puthankalam Srinivas Tomohiko Sadaoka Devin Bready Yasuko Mori Dimitris G. Placantonakis Ian Mohr Angus C. Wilson
author_facet	Daniel P. Depledge Kalanghad Puthankalam Srinivas Tomohiko Sadaoka Devin Bready Yasuko Mori Dimitris G. Placantonakis Ian Mohr Angus C. Wilson
author_sort	Daniel P. Depledge
title	Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
title_short	Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
title_full	Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
title_fullStr	Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
title_full_unstemmed	Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
title_sort	direct rna sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/62e6f7e4a2564e61a8a33f67a99d8972
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Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen

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