A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum.
The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical tetra-modular nonribosomal peptide synthetase...
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2014
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oai:doaj.org-article:6380c87e856c4595a25ae2fd2cea9b992021-11-18T08:17:29ZA non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum.1932-620310.1371/journal.pone.0098212https://doaj.org/article/6380c87e856c4595a25ae2fd2cea9b992014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24887561/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical tetra-modular nonribosomal peptide synthetase (NRPS) with microheterogenicity of all involved adenylation domains towards their respective substrates. By deleting the putative gene in combination with comparative metabolite profiling various unique cyclic and derived linear tetrapeptides were identified which were associated with this NRPS, including fungisporin. In combination with substrate predictions for each module, we propose a mechanism for a 'trans-acting' adenylation domain.Hazrat AliMarco I RiesPeter P LankhorstRob A M van der HoevenOlaf L SchoutenMarek NogaThomas HankemeierNoël N M E van PeijRoel A L BovenbergRob J VreekenArnold J M DriessenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98212 (2014) |
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| collection |
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| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Hazrat Ali Marco I Ries Peter P Lankhorst Rob A M van der Hoeven Olaf L Schouten Marek Noga Thomas Hankemeier Noël N M E van Peij Roel A L Bovenberg Rob J Vreeken Arnold J M Driessen A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| description |
The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical tetra-modular nonribosomal peptide synthetase (NRPS) with microheterogenicity of all involved adenylation domains towards their respective substrates. By deleting the putative gene in combination with comparative metabolite profiling various unique cyclic and derived linear tetrapeptides were identified which were associated with this NRPS, including fungisporin. In combination with substrate predictions for each module, we propose a mechanism for a 'trans-acting' adenylation domain. |
| format |
article |
| author |
Hazrat Ali Marco I Ries Peter P Lankhorst Rob A M van der Hoeven Olaf L Schouten Marek Noga Thomas Hankemeier Noël N M E van Peij Roel A L Bovenberg Rob J Vreeken Arnold J M Driessen |
| author_facet |
Hazrat Ali Marco I Ries Peter P Lankhorst Rob A M van der Hoeven Olaf L Schouten Marek Noga Thomas Hankemeier Noël N M E van Peij Roel A L Bovenberg Rob J Vreeken Arnold J M Driessen |
| author_sort |
Hazrat Ali |
| title |
A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| title_short |
A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| title_full |
A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| title_fullStr |
A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| title_full_unstemmed |
A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. |
| title_sort |
non-canonical nrps is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in penicillium chrysogenum. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/6380c87e856c4595a25ae2fd2cea9b99 |
| work_keys_str_mv |
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