Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression
Abstract The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory m...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/638fdda85f414409bc159c27d999af7e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:638fdda85f414409bc159c27d999af7e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:638fdda85f414409bc159c27d999af7e2021-12-02T15:23:09ZTick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression10.1038/s41598-020-80251-y2045-2322https://doaj.org/article/638fdda85f414409bc159c27d999af7e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80251-yhttps://doaj.org/toc/2045-2322Abstract The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules, such as programmed death (PD)-1 and PD-ligand 1 (PD-L1), has not been described. In this study, flow cytometric analyses revealed that Rm-saliva upregulated PD-1 expression in T cells and PD-L1 expression in CD14+ and CD11c+ cells in cattle. Additionally, Rm-saliva decreased CD69 expression in T cells and Th1 cytokine production from peripheral blood mononuclear cells. Furthermore, PD-L1 blockade increased IFN-γ production in the presence of Rm-saliva, suggesting that Rm-saliva suppresses Th1 responses via the PD-1/PD-L1 pathway. To reveal the upregulation mechanism of PD-1/PD-L1 by Rm-saliva, we analyzed the function of prostaglandin E2 (PGE2), which is known as an inducer of PD-L1 expression, in Rm-saliva. We found that Rm-saliva contained a high concentration of PGE2, and PGE2 treatment induced PD-L1 expression in CD14+ cells in vitro. Immunohistochemical analyses revealed that PGE2 and PD-L1 expression was upregulated in tick-attached skin in cattle. These data suggest that PGE2 in Rm-saliva has the potential to induce the expression of immunoinhibitory molecules in host immune cells.Yamato SajikiSatoru KonnaiYoshinori IkenakaKevin Christian Montecillo GulayAtsushi KobayashiLuís Fernando PariziBenvindo Capela JoãoKei WatariSotaro FujisawaTomohiro OkagawaNaoya MaekawaCarlos LogulloItabajara da Silva VazShiro MurataKazuhiko OhashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yamato Sajiki Satoru Konnai Yoshinori Ikenaka Kevin Christian Montecillo Gulay Atsushi Kobayashi Luís Fernando Parizi Benvindo Capela João Kei Watari Sotaro Fujisawa Tomohiro Okagawa Naoya Maekawa Carlos Logullo Itabajara da Silva Vaz Shiro Murata Kazuhiko Ohashi Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| description |
Abstract The tick Rhipicephalus microplus is a harmful parasite of cattle that causes considerable economic losses to the cattle breeding industry. Although R. microplus saliva (Rm-saliva) contains several immunosuppressants, any association between Rm-saliva and the expression of immunoinhibitory molecules, such as programmed death (PD)-1 and PD-ligand 1 (PD-L1), has not been described. In this study, flow cytometric analyses revealed that Rm-saliva upregulated PD-1 expression in T cells and PD-L1 expression in CD14+ and CD11c+ cells in cattle. Additionally, Rm-saliva decreased CD69 expression in T cells and Th1 cytokine production from peripheral blood mononuclear cells. Furthermore, PD-L1 blockade increased IFN-γ production in the presence of Rm-saliva, suggesting that Rm-saliva suppresses Th1 responses via the PD-1/PD-L1 pathway. To reveal the upregulation mechanism of PD-1/PD-L1 by Rm-saliva, we analyzed the function of prostaglandin E2 (PGE2), which is known as an inducer of PD-L1 expression, in Rm-saliva. We found that Rm-saliva contained a high concentration of PGE2, and PGE2 treatment induced PD-L1 expression in CD14+ cells in vitro. Immunohistochemical analyses revealed that PGE2 and PD-L1 expression was upregulated in tick-attached skin in cattle. These data suggest that PGE2 in Rm-saliva has the potential to induce the expression of immunoinhibitory molecules in host immune cells. |
| format |
article |
| author |
Yamato Sajiki Satoru Konnai Yoshinori Ikenaka Kevin Christian Montecillo Gulay Atsushi Kobayashi Luís Fernando Parizi Benvindo Capela João Kei Watari Sotaro Fujisawa Tomohiro Okagawa Naoya Maekawa Carlos Logullo Itabajara da Silva Vaz Shiro Murata Kazuhiko Ohashi |
| author_facet |
Yamato Sajiki Satoru Konnai Yoshinori Ikenaka Kevin Christian Montecillo Gulay Atsushi Kobayashi Luís Fernando Parizi Benvindo Capela João Kei Watari Sotaro Fujisawa Tomohiro Okagawa Naoya Maekawa Carlos Logullo Itabajara da Silva Vaz Shiro Murata Kazuhiko Ohashi |
| author_sort |
Yamato Sajiki |
| title |
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| title_short |
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| title_full |
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| title_fullStr |
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| title_full_unstemmed |
Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression |
| title_sort |
tick saliva-induced programmed death-1 and pd-ligand 1 and its related host immunosuppression |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/638fdda85f414409bc159c27d999af7e |
| work_keys_str_mv |
AT yamatosajiki ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT satorukonnai ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT yoshinoriikenaka ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT kevinchristianmontecillogulay ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT atsushikobayashi ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT luisfernandoparizi ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT benvindocapelajoao ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT keiwatari ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT sotarofujisawa ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT tomohirookagawa ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT naoyamaekawa ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT carloslogullo ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT itabajaradasilvavaz ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT shiromurata ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression AT kazuhikoohashi ticksalivainducedprogrammeddeath1andpdligand1anditsrelatedhostimmunosuppression |
| _version_ |
1718387328942080000 |