A biosensor-based framework to measure latent proteostasis capacity

A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.

Guardado en:
Detalles Bibliográficos
Autores principales: Rebecca J. Wood, Angelique R. Ormsby, Mona Radwan, Dezerae Cox, Abhishek Sharma, Tobias Vöpel, Simon Ebbinghaus, Mikael Oliveberg, Gavin E. Reid, Alex Dickson, Danny M. Hatters
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Q
Acceso en línea:https://doaj.org/article/6391112b918e4b36a3854c8ea904fe80
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!