A biosensor-based framework to measure latent proteostasis capacity
A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.
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Nature Portfolio
2018
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oai:doaj.org-article:6391112b918e4b36a3854c8ea904fe802021-12-02T14:39:27ZA biosensor-based framework to measure latent proteostasis capacity10.1038/s41467-017-02562-52041-1723https://doaj.org/article/6391112b918e4b36a3854c8ea904fe802018-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02562-5https://doaj.org/toc/2041-1723A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.Rebecca J. WoodAngelique R. OrmsbyMona RadwanDezerae CoxAbhishek SharmaTobias VöpelSimon EbbinghausMikael OlivebergGavin E. ReidAlex DicksonDanny M. HattersNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Rebecca J. Wood Angelique R. Ormsby Mona Radwan Dezerae Cox Abhishek Sharma Tobias Vöpel Simon Ebbinghaus Mikael Oliveberg Gavin E. Reid Alex Dickson Danny M. Hatters A biosensor-based framework to measure latent proteostasis capacity |
| description |
A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation. |
| format |
article |
| author |
Rebecca J. Wood Angelique R. Ormsby Mona Radwan Dezerae Cox Abhishek Sharma Tobias Vöpel Simon Ebbinghaus Mikael Oliveberg Gavin E. Reid Alex Dickson Danny M. Hatters |
| author_facet |
Rebecca J. Wood Angelique R. Ormsby Mona Radwan Dezerae Cox Abhishek Sharma Tobias Vöpel Simon Ebbinghaus Mikael Oliveberg Gavin E. Reid Alex Dickson Danny M. Hatters |
| author_sort |
Rebecca J. Wood |
| title |
A biosensor-based framework to measure latent proteostasis capacity |
| title_short |
A biosensor-based framework to measure latent proteostasis capacity |
| title_full |
A biosensor-based framework to measure latent proteostasis capacity |
| title_fullStr |
A biosensor-based framework to measure latent proteostasis capacity |
| title_full_unstemmed |
A biosensor-based framework to measure latent proteostasis capacity |
| title_sort |
biosensor-based framework to measure latent proteostasis capacity |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/6391112b918e4b36a3854c8ea904fe80 |
| work_keys_str_mv |
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