Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets

When using the Himar1 transposon to create transposon insertion mutant libraries, it is known that the transposon is restricted to insertions at TA dinucleotide sites throughout the genome, and the absence of insertions is used to infer which genes are essential (or conditionally essential) in a ba...

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Auteurs principaux: Sanjeevani Choudhery, A. Jacob Brown, Chidiebere Akusobi, Eric J. Rubin, Christopher M. Sassetti, Thomas R. Ioerger
Format: article
Langue:EN
Publié: American Society for Microbiology 2021
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Accès en ligne:https://doaj.org/article/639d8bc4ff574b599af7ecfa2b2035f1
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