Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets

When using the Himar1 transposon to create transposon insertion mutant libraries, it is known that the transposon is restricted to insertions at TA dinucleotide sites throughout the genome, and the absence of insertions is used to infer which genes are essential (or conditionally essential) in a ba...

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Autores principales: Sanjeevani Choudhery, A. Jacob Brown, Chidiebere Akusobi, Eric J. Rubin, Christopher M. Sassetti, Thomas R. Ioerger
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2021
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Acceso en línea:https://doaj.org/article/639d8bc4ff574b599af7ecfa2b2035f1
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