Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets

When using the Himar1 transposon to create transposon insertion mutant libraries, it is known that the transposon is restricted to insertions at TA dinucleotide sites throughout the genome, and the absence of insertions is used to infer which genes are essential (or conditionally essential) in a ba...

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Autores principales:	Sanjeevani Choudhery, A. Jacob Brown, Chidiebere Akusobi, Eric J. Rubin, Christopher M. Sassetti, Thomas R. Ioerger
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/639d8bc4ff574b599af7ecfa2b2035f1
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id	oai:doaj.org-article:639d8bc4ff574b599af7ecfa2b2035f1
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spelling	oai:doaj.org-article:639d8bc4ff574b599af7ecfa2b2035f12021-12-02T17:48:14ZModeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets2379-507710.1128/mSystems.00876-21https://doaj.org/article/639d8bc4ff574b599af7ecfa2b2035f12021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00876-21https://doaj.org/toc/2379-5077 When using the Himar1 transposon to create transposon insertion mutant libraries, it is known that the transposon is restricted to insertions at TA dinucleotide sites throughout the genome, and the absence of insertions is used to infer which genes are essential (or conditionally essential) in a bacterial organism. It is widely assumed that insertions in nonessential regions are otherwise random, and this assumption is used as the basis of several methods for statistical analysis of TnSeq data.Sanjeevani ChoudheryA. Jacob BrownChidiebere AkusobiEric J. RubinChristopher M. SassettiThomas R. IoergerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sanjeevani Choudhery A. Jacob Brown Chidiebere Akusobi Eric J. Rubin Christopher M. Sassetti Thomas R. Ioerger Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
description	When using the Himar1 transposon to create transposon insertion mutant libraries, it is known that the transposon is restricted to insertions at TA dinucleotide sites throughout the genome, and the absence of insertions is used to infer which genes are essential (or conditionally essential) in a bacterial organism. It is widely assumed that insertions in nonessential regions are otherwise random, and this assumption is used as the basis of several methods for statistical analysis of TnSeq data.
format	article
author	Sanjeevani Choudhery A. Jacob Brown Chidiebere Akusobi Eric J. Rubin Christopher M. Sassetti Thomas R. Ioerger
author_facet	Sanjeevani Choudhery A. Jacob Brown Chidiebere Akusobi Eric J. Rubin Christopher M. Sassetti Thomas R. Ioerger
author_sort	Sanjeevani Choudhery
title	Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
title_short	Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
title_full	Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
title_fullStr	Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
title_full_unstemmed	Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets
title_sort	modeling site-specific nucleotide biases affecting himar1 transposon insertion frequencies in tnseq data sets
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/639d8bc4ff574b599af7ecfa2b2035f1
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Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets

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