Detecting stoichiometry of macromolecular complexes in live cells using FRET

Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin bindin...

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Autores principales: Manu Ben-Johny, Daniel N. Yue, David T. Yue
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/64c4b3b1f151448da9dc8c818a6f3f7e
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