Detecting stoichiometry of macromolecular complexes in live cells using FRET

Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin bindin...

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Autores principales:	Manu Ben-Johny, Daniel N. Yue, David T. Yue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/64c4b3b1f151448da9dc8c818a6f3f7e
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spelling	oai:doaj.org-article:64c4b3b1f151448da9dc8c818a6f3f7e2021-12-02T14:38:33ZDetecting stoichiometry of macromolecular complexes in live cells using FRET10.1038/ncomms137092041-1723https://doaj.org/article/64c4b3b1f151448da9dc8c818a6f3f7e2016-12-01T00:00:00Zhttps://doi.org/10.1038/ncomms13709https://doaj.org/toc/2041-1723Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.Manu Ben-JohnyDaniel N. YueDavid T. YueNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Manu Ben-Johny Daniel N. Yue David T. Yue Detecting stoichiometry of macromolecular complexes in live cells using FRET
description	Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.
format	article
author	Manu Ben-Johny Daniel N. Yue David T. Yue
author_facet	Manu Ben-Johny Daniel N. Yue David T. Yue
author_sort	Manu Ben-Johny
title	Detecting stoichiometry of macromolecular complexes in live cells using FRET
title_short	Detecting stoichiometry of macromolecular complexes in live cells using FRET
title_full	Detecting stoichiometry of macromolecular complexes in live cells using FRET
title_fullStr	Detecting stoichiometry of macromolecular complexes in live cells using FRET
title_full_unstemmed	Detecting stoichiometry of macromolecular complexes in live cells using FRET
title_sort	detecting stoichiometry of macromolecular complexes in live cells using fret
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/64c4b3b1f151448da9dc8c818a6f3f7e
work_keys_str_mv	AT manubenjohny detectingstoichiometryofmacromolecularcomplexesinlivecellsusingfret AT danielnyue detectingstoichiometryofmacromolecularcomplexesinlivecellsusingfret AT davidtyue detectingstoichiometryofmacromolecularcomplexesinlivecellsusingfret
_version_	1718390954089512960

Detecting stoichiometry of macromolecular complexes in live cells using FRET

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