Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.

Recent studies have demonstrated the effectiveness of vaccine delivery to the skin by vaccine-coated microneedles; however there is little information on the effects of adjuvants using this approach for vaccination. Here we investigate the use of TLR ligands as adjuvants with skin-based delivery of...

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Autores principales:	William C Weldon, Vladimir G Zarnitsyn, E Stein Esser, Misha T Taherbhai, Dimitrios G Koutsonanos, Elena V Vassilieva, Ioanna Skountzou, Mark R Prausnitz, Richard W Compans
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64fbf7d8229249eeb0167bf3d49491d8
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spelling	oai:doaj.org-article:64fbf7d8229249eeb0167bf3d49491d82021-11-18T07:11:07ZEffect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.1932-620310.1371/journal.pone.0041501https://doaj.org/article/64fbf7d8229249eeb0167bf3d49491d82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848514/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies have demonstrated the effectiveness of vaccine delivery to the skin by vaccine-coated microneedles; however there is little information on the effects of adjuvants using this approach for vaccination. Here we investigate the use of TLR ligands as adjuvants with skin-based delivery of influenza subunit vaccine. BALB/c mice received 1 µg of monovalent H1N1 subunit vaccine alone or with 1 µg of imiquimod or poly(I:C) individually or in combination via coated microneedle patches inserted into the skin. Poly(I:C) adjuvanted subunit influenza vaccine induced similar antigen-specific immune responses compared to vaccine alone when delivered to the skin by microneedles. However, imiquimod-adjuvanted vaccine elicited higher levels of serum IgG2a antibodies and increased hemagglutination inhibition titers compared to vaccine alone, suggesting enhanced induction of functional antibodies. In addition, imiquimod-adjuvanted vaccine induced a robust IFN-γ cellular response. These responses correlated with improved protection compared to influenza subunit vaccine alone, as well as reduced viral replication and production of pro-inflammatory cytokines in the lungs. The finding that microneedle delivery of imiquimod with influenza subunit vaccine induces improved immune responses compared to vaccine alone supports the use of TLR7 ligands as adjuvants for skin-based influenza vaccines.William C WeldonVladimir G ZarnitsynE Stein EsserMisha T TaherbhaiDimitrios G KoutsonanosElena V VassilievaIoanna SkountzouMark R PrausnitzRichard W CompansPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41501 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q William C Weldon Vladimir G Zarnitsyn E Stein Esser Misha T Taherbhai Dimitrios G Koutsonanos Elena V Vassilieva Ioanna Skountzou Mark R Prausnitz Richard W Compans Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
description	Recent studies have demonstrated the effectiveness of vaccine delivery to the skin by vaccine-coated microneedles; however there is little information on the effects of adjuvants using this approach for vaccination. Here we investigate the use of TLR ligands as adjuvants with skin-based delivery of influenza subunit vaccine. BALB/c mice received 1 µg of monovalent H1N1 subunit vaccine alone or with 1 µg of imiquimod or poly(I:C) individually or in combination via coated microneedle patches inserted into the skin. Poly(I:C) adjuvanted subunit influenza vaccine induced similar antigen-specific immune responses compared to vaccine alone when delivered to the skin by microneedles. However, imiquimod-adjuvanted vaccine elicited higher levels of serum IgG2a antibodies and increased hemagglutination inhibition titers compared to vaccine alone, suggesting enhanced induction of functional antibodies. In addition, imiquimod-adjuvanted vaccine induced a robust IFN-γ cellular response. These responses correlated with improved protection compared to influenza subunit vaccine alone, as well as reduced viral replication and production of pro-inflammatory cytokines in the lungs. The finding that microneedle delivery of imiquimod with influenza subunit vaccine induces improved immune responses compared to vaccine alone supports the use of TLR7 ligands as adjuvants for skin-based influenza vaccines.
format	article
author	William C Weldon Vladimir G Zarnitsyn E Stein Esser Misha T Taherbhai Dimitrios G Koutsonanos Elena V Vassilieva Ioanna Skountzou Mark R Prausnitz Richard W Compans
author_facet	William C Weldon Vladimir G Zarnitsyn E Stein Esser Misha T Taherbhai Dimitrios G Koutsonanos Elena V Vassilieva Ioanna Skountzou Mark R Prausnitz Richard W Compans
author_sort	William C Weldon
title	Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
title_short	Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
title_full	Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
title_fullStr	Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
title_full_unstemmed	Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
title_sort	effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/64fbf7d8229249eeb0167bf3d49491d8
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Effect of adjuvants on responses to skin immunization by microneedles coated with influenza subunit vaccine.

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