Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes

ABSTRACT We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosom...

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Autores principales:	Anna Nenarokova, Kristína Záhonová, Marija Krasilnikova, Ondřej Gahura, Richard McCulloch, Alena Zíková, Vyacheslav Yurchenko, Julius Lukeš
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	DNA repair genome size parasite Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/654d7dbb7b3644f2bb0e34c89954906e
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spelling	oai:doaj.org-article:654d7dbb7b3644f2bb0e34c89954906e2021-11-15T16:22:11ZCauses and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes10.1128/mBio.01541-192150-7511https://doaj.org/article/654d7dbb7b3644f2bb0e34c89954906e2019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01541-19https://doaj.org/toc/2150-7511ABSTRACT We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosomatid flagellates that has lost Ku70 and Ku80, the core C-NHEJ components, and accumulated numerous insertions in many protein-coding genes. We propose a correlation between these two phenomena and discuss the possible impact of the C-NHEJ loss on genome evolution and transition to the parasitic lifestyle. IMPORTANCE Parasites tend to evolve small and compact genomes, generally endowed with a high mutation rate, compared with those of their free-living relatives. However, the mechanisms by which they achieve these features, independently in unrelated lineages, remain largely unknown. We argue that the loss of the classical nonhomologous end joining pathway components may be one of the crucial steps responsible for characteristic features of parasite genomes.Anna NenarokovaKristína ZáhonováMarija KrasilnikovaOndřej GahuraRichard McCullochAlena ZíkováVyacheslav YurchenkoJulius LukešAmerican Society for MicrobiologyarticleDNA repairgenome sizeparasiteMicrobiologyQR1-502ENmBio, Vol 10, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA repair genome size parasite Microbiology QR1-502
spellingShingle	DNA repair genome size parasite Microbiology QR1-502 Anna Nenarokova Kristína Záhonová Marija Krasilnikova Ondřej Gahura Richard McCulloch Alena Zíková Vyacheslav Yurchenko Julius Lukeš Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
description	ABSTRACT We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosomatid flagellates that has lost Ku70 and Ku80, the core C-NHEJ components, and accumulated numerous insertions in many protein-coding genes. We propose a correlation between these two phenomena and discuss the possible impact of the C-NHEJ loss on genome evolution and transition to the parasitic lifestyle. IMPORTANCE Parasites tend to evolve small and compact genomes, generally endowed with a high mutation rate, compared with those of their free-living relatives. However, the mechanisms by which they achieve these features, independently in unrelated lineages, remain largely unknown. We argue that the loss of the classical nonhomologous end joining pathway components may be one of the crucial steps responsible for characteristic features of parasite genomes.
format	article
author	Anna Nenarokova Kristína Záhonová Marija Krasilnikova Ondřej Gahura Richard McCulloch Alena Zíková Vyacheslav Yurchenko Julius Lukeš
author_facet	Anna Nenarokova Kristína Záhonová Marija Krasilnikova Ondřej Gahura Richard McCulloch Alena Zíková Vyacheslav Yurchenko Julius Lukeš
author_sort	Anna Nenarokova
title	Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
title_short	Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
title_full	Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
title_fullStr	Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
title_full_unstemmed	Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
title_sort	causes and effects of loss of classical nonhomologous end joining pathway in parasitic eukaryotes
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/654d7dbb7b3644f2bb0e34c89954906e
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Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes

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