Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

Systemic investigation of the bacterial genome is essential for both basic microbiology and bioengineering. Here the authors demonstrate CRISPRi pooled screening as a high-throughput tool for identifying gene and phenotype associations in bacteria.

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Autores principales: Tianmin Wang, Changge Guan, Jiahui Guo, Bing Liu, Yinan Wu, Zhen Xie, Chong Zhang, Xin-Hui Xing
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/65cefdd2f6ff45aeb52febb0e268d6b1
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