Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

Systemic investigation of the bacterial genome is essential for both basic microbiology and bioengineering. Here the authors demonstrate CRISPRi pooled screening as a high-throughput tool for identifying gene and phenotype associations in bacteria.

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Autores principales:	Tianmin Wang, Changge Guan, Jiahui Guo, Bing Liu, Yinan Wu, Zhen Xie, Chong Zhang, Xin-Hui Xing
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/65cefdd2f6ff45aeb52febb0e268d6b1
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spelling	oai:doaj.org-article:65cefdd2f6ff45aeb52febb0e268d6b12021-12-02T15:33:43ZPooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance10.1038/s41467-018-04899-x2041-1723https://doaj.org/article/65cefdd2f6ff45aeb52febb0e268d6b12018-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04899-xhttps://doaj.org/toc/2041-1723Systemic investigation of the bacterial genome is essential for both basic microbiology and bioengineering. Here the authors demonstrate CRISPRi pooled screening as a high-throughput tool for identifying gene and phenotype associations in bacteria.Tianmin WangChangge GuanJiahui GuoBing LiuYinan WuZhen XieChong ZhangXin-Hui XingNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tianmin Wang Changge Guan Jiahui Guo Bing Liu Yinan Wu Zhen Xie Chong Zhang Xin-Hui Xing Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
description	Systemic investigation of the bacterial genome is essential for both basic microbiology and bioengineering. Here the authors demonstrate CRISPRi pooled screening as a high-throughput tool for identifying gene and phenotype associations in bacteria.
format	article
author	Tianmin Wang Changge Guan Jiahui Guo Bing Liu Yinan Wu Zhen Xie Chong Zhang Xin-Hui Xing
author_facet	Tianmin Wang Changge Guan Jiahui Guo Bing Liu Yinan Wu Zhen Xie Chong Zhang Xin-Hui Xing
author_sort	Tianmin Wang
title	Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
title_short	Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
title_full	Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
title_fullStr	Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
title_full_unstemmed	Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
title_sort	pooled crispr interference screening enables genome-scale functional genomics study in bacteria with superior performance
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/65cefdd2f6ff45aeb52febb0e268d6b1
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_version_	1718387028128694272

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

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