The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.
Rhodopsin mistrafficking can cause photoreceptor (PR) degeneration. Upon light exposure, activated rhodopsin 1 (Rh1) in Drosophila PRs is internalized via endocytosis and degraded in lysosomes. Whether internalized Rh1 can be recycled is unknown. Here, we show that the retromer complex is expressed...
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2014
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oai:doaj.org-article:65e1429ffb374718abe3785f3e9b39d52021-11-18T05:37:29ZThe retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.1544-91731545-788510.1371/journal.pbio.1001847https://doaj.org/article/65e1429ffb374718abe3785f3e9b39d52014-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24781186/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Rhodopsin mistrafficking can cause photoreceptor (PR) degeneration. Upon light exposure, activated rhodopsin 1 (Rh1) in Drosophila PRs is internalized via endocytosis and degraded in lysosomes. Whether internalized Rh1 can be recycled is unknown. Here, we show that the retromer complex is expressed in PRs where it is required for recycling endocytosed Rh1 upon light stimulation. In the absence of subunits of the retromer, Rh1 is processed in the endolysosomal pathway, leading to a dramatic increase in late endosomes, lysosomes, and light-dependent PR degeneration. Reducing Rh1 endocytosis or Rh1 levels in retromer mutants alleviates PR degeneration. In addition, increasing retromer abundance suppresses degenerative phenotypes of mutations that affect the endolysosomal system. Finally, expressing human Vps26 suppresses PR degeneration in Vps26 mutant PRs. We propose that the retromer plays a conserved role in recycling rhodopsins to maintain PR function and integrity.Shiuan WangKai Li TanMelina A AgostoBo XiongShinya YamamotoHector SandovalManish JaiswalVafa BayatKe ZhangWu-Lin CharngGabriela DavidLita DuraineKartik VenkatachalamTheodore G WenselHugo J BellenPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 4, p e1001847 (2014) |
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DOAJ |
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DOAJ |
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Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Shiuan Wang Kai Li Tan Melina A Agosto Bo Xiong Shinya Yamamoto Hector Sandoval Manish Jaiswal Vafa Bayat Ke Zhang Wu-Lin Charng Gabriela David Lita Duraine Kartik Venkatachalam Theodore G Wensel Hugo J Bellen The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| description |
Rhodopsin mistrafficking can cause photoreceptor (PR) degeneration. Upon light exposure, activated rhodopsin 1 (Rh1) in Drosophila PRs is internalized via endocytosis and degraded in lysosomes. Whether internalized Rh1 can be recycled is unknown. Here, we show that the retromer complex is expressed in PRs where it is required for recycling endocytosed Rh1 upon light stimulation. In the absence of subunits of the retromer, Rh1 is processed in the endolysosomal pathway, leading to a dramatic increase in late endosomes, lysosomes, and light-dependent PR degeneration. Reducing Rh1 endocytosis or Rh1 levels in retromer mutants alleviates PR degeneration. In addition, increasing retromer abundance suppresses degenerative phenotypes of mutations that affect the endolysosomal system. Finally, expressing human Vps26 suppresses PR degeneration in Vps26 mutant PRs. We propose that the retromer plays a conserved role in recycling rhodopsins to maintain PR function and integrity. |
| format |
article |
| author |
Shiuan Wang Kai Li Tan Melina A Agosto Bo Xiong Shinya Yamamoto Hector Sandoval Manish Jaiswal Vafa Bayat Ke Zhang Wu-Lin Charng Gabriela David Lita Duraine Kartik Venkatachalam Theodore G Wensel Hugo J Bellen |
| author_facet |
Shiuan Wang Kai Li Tan Melina A Agosto Bo Xiong Shinya Yamamoto Hector Sandoval Manish Jaiswal Vafa Bayat Ke Zhang Wu-Lin Charng Gabriela David Lita Duraine Kartik Venkatachalam Theodore G Wensel Hugo J Bellen |
| author_sort |
Shiuan Wang |
| title |
The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| title_short |
The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| title_full |
The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| title_fullStr |
The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| title_full_unstemmed |
The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| title_sort |
retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/65e1429ffb374718abe3785f3e9b39d5 |
| work_keys_str_mv |
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