Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Abstract The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added...

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Autores principales: Miao Zhang, Harriëtte Oldenhof, Bulat Sydykov, Judith Bigalk, Harald Sieme, Willem F. Wolkers
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/6818fc6151594e72a6ebc0ad9c7eefd8
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