Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity
Abstract The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added...
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Autores principales: | Miao Zhang, Harriëtte Oldenhof, Bulat Sydykov, Judith Bigalk, Harald Sieme, Willem F. Wolkers |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
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Materias: | |
Acceso en línea: | https://doaj.org/article/6818fc6151594e72a6ebc0ad9c7eefd8 |
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