Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.

Guardado en:

Detalles Bibliográficos
Autores principales:	Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:685c8caed9c1480ca04b435e72570dc1
record_format	dspace
spelling	oai:doaj.org-article:685c8caed9c1480ca04b435e72570dc12021-12-02T17:32:04ZPairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells10.1038/s41467-018-05391-22041-1723https://doaj.org/article/685c8caed9c1480ca04b435e72570dc12018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05391-2https://doaj.org/toc/2041-1723A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.Josh TyckoLuis A. BarreraNicholas C. HustonAri E. FriedlandXuebing WuJonathan S. GootenbergOmar O. AbudayyehVic E. MyerChristopher J. WilsonPatrick D. HsuNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Josh Tycko Luis A. Barrera Nicholas C. Huston Ari E. Friedland Xuebing Wu Jonathan S. Gootenberg Omar O. Abudayyeh Vic E. Myer Christopher J. Wilson Patrick D. Hsu Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
description	A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.
format	article
author	Josh Tycko Luis A. Barrera Nicholas C. Huston Ari E. Friedland Xuebing Wu Jonathan S. Gootenberg Omar O. Abudayyeh Vic E. Myer Christopher J. Wilson Patrick D. Hsu
author_facet	Josh Tycko Luis A. Barrera Nicholas C. Huston Ari E. Friedland Xuebing Wu Jonathan S. Gootenberg Omar O. Abudayyeh Vic E. Myer Christopher J. Wilson Patrick D. Hsu
author_sort	Josh Tycko
title	Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
title_short	Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
title_full	Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
title_fullStr	Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
title_full_unstemmed	Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
title_sort	pairwise library screen systematically interrogates staphylococcus aureus cas9 specificity in human cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
work_keys_str_mv	AT joshtycko pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT luisabarrera pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT nicholaschuston pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT ariefriedland pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT xuebingwu pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT jonathansgootenberg pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT omaroabudayyeh pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT vicemyer pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT christopherjwilson pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells AT patrickdhsu pairwiselibraryscreensystematicallyinterrogatesstaphylococcusaureuscas9specificityinhumancells
_version_	1718380433610113024

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Ejemplares similares