A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain

Abstract The micronucleus is known to be a biomarker for genomic instability, which is a hallmark of tumors and aging. Normally, micronuclei are produced by segregation errors and mechanical stresses arising from dividing or migrating cells, leading to activation of the innate immune response pathwa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sarasa Yano, Kaito Akiyama, Rio Tsuchiya, Hikari Kubotani, Tomoki Chiba, Takeshi Nagata, Fuminori Tsuruta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/692244364ba24204a0d4329f4880b541
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:692244364ba24204a0d4329f4880b541
record_format	dspace
spelling	oai:doaj.org-article:692244364ba24204a0d4329f4880b5412021-12-02T17:23:47ZA MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain10.1038/s41598-021-97640-62045-2322https://doaj.org/article/692244364ba24204a0d4329f4880b5412021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97640-6https://doaj.org/toc/2045-2322Abstract The micronucleus is known to be a biomarker for genomic instability, which is a hallmark of tumors and aging. Normally, micronuclei are produced by segregation errors and mechanical stresses arising from dividing or migrating cells, leading to activation of the innate immune response pathway. Although micronuclei often emerge in damaged tissues, the quantitative procedure for analyzing micronuclei accurately has been problematic. Here, we introduce a novel MATLAB-based program for quantifying micronuclei (CAMDi: calculating automatic micronuclei distinction) in vitro and in vivo. CAMDi is adaptable to various experimental imaging techniques and is useful for obtaining reproducible data. CAMDi enables us to measure the accurate size of micronuclei from the three-dimensional images. Using CAMDi, we revealed a novel link between the emergence of micronuclei and neuroinflammation. We found that inflammatory stimulation does not increase the number of micronuclei in primary neurons. On the other hand, the administration of lipopolysaccharide into mice slightly increases micronuclei formation in neurons of the hippocampus region. These findings demonstrate that neuronal micronuclei formations are induced by an inflammatory response in a non-cell-autonomous manner. We provide a novel tool, CAMDi, to quantify micronuclei and demonstrate that neuronal micronuclei are produced not only by the cell-autonomous process but also by the intercellular communication associated with neuroinflammation in vivo.Sarasa YanoKaito AkiyamaRio TsuchiyaHikari KubotaniTomoki ChibaTakeshi NagataFuminori TsurutaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarasa Yano Kaito Akiyama Rio Tsuchiya Hikari Kubotani Tomoki Chiba Takeshi Nagata Fuminori Tsuruta A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
description	Abstract The micronucleus is known to be a biomarker for genomic instability, which is a hallmark of tumors and aging. Normally, micronuclei are produced by segregation errors and mechanical stresses arising from dividing or migrating cells, leading to activation of the innate immune response pathway. Although micronuclei often emerge in damaged tissues, the quantitative procedure for analyzing micronuclei accurately has been problematic. Here, we introduce a novel MATLAB-based program for quantifying micronuclei (CAMDi: calculating automatic micronuclei distinction) in vitro and in vivo. CAMDi is adaptable to various experimental imaging techniques and is useful for obtaining reproducible data. CAMDi enables us to measure the accurate size of micronuclei from the three-dimensional images. Using CAMDi, we revealed a novel link between the emergence of micronuclei and neuroinflammation. We found that inflammatory stimulation does not increase the number of micronuclei in primary neurons. On the other hand, the administration of lipopolysaccharide into mice slightly increases micronuclei formation in neurons of the hippocampus region. These findings demonstrate that neuronal micronuclei formations are induced by an inflammatory response in a non-cell-autonomous manner. We provide a novel tool, CAMDi, to quantify micronuclei and demonstrate that neuronal micronuclei are produced not only by the cell-autonomous process but also by the intercellular communication associated with neuroinflammation in vivo.
format	article
author	Sarasa Yano Kaito Akiyama Rio Tsuchiya Hikari Kubotani Tomoki Chiba Takeshi Nagata Fuminori Tsuruta
author_facet	Sarasa Yano Kaito Akiyama Rio Tsuchiya Hikari Kubotani Tomoki Chiba Takeshi Nagata Fuminori Tsuruta
author_sort	Sarasa Yano
title	A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
title_short	A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
title_full	A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
title_fullStr	A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
title_full_unstemmed	A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
title_sort	matlab-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/692244364ba24204a0d4329f4880b541
work_keys_str_mv	AT sarasayano amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT kaitoakiyama amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT riotsuchiya amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT hikarikubotani amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT tomokichiba amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT takeshinagata amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT fuminoritsuruta amatlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT sarasayano matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT kaitoakiyama matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT riotsuchiya matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT hikarikubotani matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT tomokichiba matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT takeshinagata matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain AT fuminoritsuruta matlabbasedprogramforthreedimensionalquantitativeanalysisofmicronucleirevealsthatneuroinflammationinducesmicronucleiformationinthebrain
_version_	1718380974685814784

A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the brain

Ejemplares similares