Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines

Abstract We describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture...

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Autores principales: Mohaned Ahmed, Haneen A. Basheer, Jose M. Ayuso, Djevdet Ahmet, Marco Mazzini, Roshan Patel, Steven D. Shnyder, Victoria Vinader, Kamyar Afarinkia
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/696870599d204173b0f1ab997124d247
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