Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation

Sepsis remains a major cause of mortality and morbidity in intensive care units. Dexmedetomidine (DEX) has been reported to attenuate cecal ligation perforation (CLP)-stimulated acute lung injury (ALI) by downregulating high-mobility group protein 1 (HMGB1) and receptor for advanced glycation end pr...

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Autores principales:	Huaqin Sun, Hongyi Hu, Xiaoping Xu, Mingsun Fang, Tao Tao, Zhehao Liang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	dexmedetomidine acute lung injury hmgb1 rage nf-κb pyroptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/6ac67c1f8d094b458a6af15a2e0a6b7e
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spelling	oai:doaj.org-article:6ac67c1f8d094b458a6af15a2e0a6b7e2021-11-11T14:23:43ZProtective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation2165-59792165-598710.1080/21655979.2021.2000723https://doaj.org/article/6ac67c1f8d094b458a6af15a2e0a6b7e2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000723https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Sepsis remains a major cause of mortality and morbidity in intensive care units. Dexmedetomidine (DEX) has been reported to attenuate cecal ligation perforation (CLP)-stimulated acute lung injury (ALI) by downregulating high-mobility group protein 1 (HMGB1) and receptor for advanced glycation end products (RAGE) expressions. This study aimed to further investigate the specific mechanisms of RAGE and its potential-related mechanisms of DEX on ALI models in vitro and in vivo. The in vitro and in vivo ALI models were established by lipopolysaccharide (LPS) treatment in MLE-12 cells and CLP in mice, respectively. The effect of DEX on pathological alteration was investigated by HE staining. Thereafter, the myeloperoxidase (MPO) activity and inflammatory cytokine levels were respectively detected to assess the lung injury of mice using commercial kits. The expression levels of HMGB1, RAGE, nuclear factor-κB (NF-κB), and pyroptosis-related molecules were detected by quantitative real-time polymerase chain reaction and western blot. HE staining showed that lung injury, increased inflammatory cell infiltration, and lung permeability was found in the ALI mice, and DEX treatment significantly attenuated lung tissue damage induced by CLP. The MPO activity and inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1β, and NLR family pyrin domain-containing 3) levels were also significantly reduced after DEX treatment compared with those in the ALI mice. Moreover, DEX activated the HMGB1/RAGE/NF-κB pathway and upregulated the pyroptosis-related proteins. However, the protective DEX effect was impaired by RAGE overexpression in ALI mice and MLE-12 cells. In addition, DEX treatment significantly suppressed HMGB1 translocation from the nucleus region to the cytoplasm, and this effect was reversed by RAGE overexpression. These findings suggested that DEX may be a useful ALI treatment, and the protective effects on ALI mice may be through the inhibition of the HMGB1/RAGE/NF-κB pathway and cell pyroptosis.Huaqin SunHongyi HuXiaoping XuMingsun FangTao TaoZhehao LiangTaylor & Francis Grouparticledexmedetomidineacute lung injuryhmgb1ragenf-κbpyroptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dexmedetomidine acute lung injury hmgb1 rage nf-κb pyroptosis Biotechnology TP248.13-248.65
spellingShingle	dexmedetomidine acute lung injury hmgb1 rage nf-κb pyroptosis Biotechnology TP248.13-248.65 Huaqin Sun Hongyi Hu Xiaoping Xu Mingsun Fang Tao Tao Zhehao Liang Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
description	Sepsis remains a major cause of mortality and morbidity in intensive care units. Dexmedetomidine (DEX) has been reported to attenuate cecal ligation perforation (CLP)-stimulated acute lung injury (ALI) by downregulating high-mobility group protein 1 (HMGB1) and receptor for advanced glycation end products (RAGE) expressions. This study aimed to further investigate the specific mechanisms of RAGE and its potential-related mechanisms of DEX on ALI models in vitro and in vivo. The in vitro and in vivo ALI models were established by lipopolysaccharide (LPS) treatment in MLE-12 cells and CLP in mice, respectively. The effect of DEX on pathological alteration was investigated by HE staining. Thereafter, the myeloperoxidase (MPO) activity and inflammatory cytokine levels were respectively detected to assess the lung injury of mice using commercial kits. The expression levels of HMGB1, RAGE, nuclear factor-κB (NF-κB), and pyroptosis-related molecules were detected by quantitative real-time polymerase chain reaction and western blot. HE staining showed that lung injury, increased inflammatory cell infiltration, and lung permeability was found in the ALI mice, and DEX treatment significantly attenuated lung tissue damage induced by CLP. The MPO activity and inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1β, and NLR family pyrin domain-containing 3) levels were also significantly reduced after DEX treatment compared with those in the ALI mice. Moreover, DEX activated the HMGB1/RAGE/NF-κB pathway and upregulated the pyroptosis-related proteins. However, the protective DEX effect was impaired by RAGE overexpression in ALI mice and MLE-12 cells. In addition, DEX treatment significantly suppressed HMGB1 translocation from the nucleus region to the cytoplasm, and this effect was reversed by RAGE overexpression. These findings suggested that DEX may be a useful ALI treatment, and the protective effects on ALI mice may be through the inhibition of the HMGB1/RAGE/NF-κB pathway and cell pyroptosis.
format	article
author	Huaqin Sun Hongyi Hu Xiaoping Xu Mingsun Fang Tao Tao Zhehao Liang
author_facet	Huaqin Sun Hongyi Hu Xiaoping Xu Mingsun Fang Tao Tao Zhehao Liang
author_sort	Huaqin Sun
title	Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
title_short	Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
title_full	Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
title_fullStr	Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
title_full_unstemmed	Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation
title_sort	protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through hmgb1/rage pathway regulation and pyroptosis activation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/6ac67c1f8d094b458a6af15a2e0a6b7e
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Protective effect of dexmedetomidine in cecal ligation perforation-induced acute lung injury through HMGB1/RAGE pathway regulation and pyroptosis activation

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