The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci

In the present study, the effect of B. subtilis (107cfu/gm) supplemented to the basal diet of two groups of New Zealand rabbit of 28 days (n=15/group) on the count of commensal microflora ( Bifidobacteria and Lactobacilli spp.) and some opportunistic pathogens (E. coli and C. perfringens type A) in...

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Autores principales:	Eman, F. Farag, G. A. Abdel-Alim
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	rabbit probiotics b. subtilis c. perfringens e. coli caeci Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.117995 https://doaj.org/article/6b5933e9b0d548c38d852c8a7bac8e05
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spelling	oai:doaj.org-article:6b5933e9b0d548c38d852c8a7bac8e052021-12-02T14:02:57ZThe Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caecihttps://dx.doi.org/10.21608/javs.2020.1179951687-40722090-3308https://doaj.org/article/6b5933e9b0d548c38d852c8a7bac8e052020-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_117995.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308In the present study, the effect of B. subtilis (107cfu/gm) supplemented to the basal diet of two groups of New Zealand rabbit of 28 days (n=15/group) on the count of commensal microflora ( Bifidobacteria and Lactobacilli spp.) and some opportunistic pathogens (E. coli and C. perfringens type A) in the rabbit caeci was investigated. Results indicated that the count of Bifidobacteria and Lactobacilli spp. are significantly increased at all intervals (7, 14, 28 and 40 days post supplementation) in the supplemented group compared with non-supplemented group with P< 0.001. On the other hand, there is a significant decrease in the count of E. coli and C. perfringens specially at 40 days post supplementation from (5.8 log 10 cfu ±0.06) to (3.1 log 10 cfu ±0.07) and from (4.8 log 10 cfu ±0.09) to (2.3 log 10 cfu ±0.09) respectively with P< 0.001. B. subtilis proved to be tolerant to the simulated gastric and intestinal juice for one hour (100% tolerance). However, after two hours, the tolerance to the gastric juice decreased to 70%, with no effect of the artificial intestinal juice on the viability of the organism. In vitro, the antimicrobial effect of B. subtilis on C. perfringens type A by well diffusion method showed an inhibition zone of 10 mm. Results of in vitro effect of B. subtilis on C. perfringens count showed that at 103 dilutions of C. perfringens, its count was reduced 2.1×105 to 3×104 after adding B. subtilis, while at dilution of 102, the count was reduced from 2.4×105 to 7×103cfu/ml after adding B. subtilis. It could be concluded that B. subtilis can be used as a probiotic in rabbits ration due to its ability to increase the commensal microflora count beside its antibacterial effect against some opportunistic pathogens.Eman, F. FaragG. A. Abdel-AlimEgyptian Society for Animal Managementarticlerabbitprobioticsb. subtilisc. perfringense. colicaeciZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 4, Pp 10-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	rabbit probiotics b. subtilis c. perfringens e. coli caeci Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	rabbit probiotics b. subtilis c. perfringens e. coli caeci Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Eman, F. Farag G. A. Abdel-Alim The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
description	In the present study, the effect of B. subtilis (107cfu/gm) supplemented to the basal diet of two groups of New Zealand rabbit of 28 days (n=15/group) on the count of commensal microflora ( Bifidobacteria and Lactobacilli spp.) and some opportunistic pathogens (E. coli and C. perfringens type A) in the rabbit caeci was investigated. Results indicated that the count of Bifidobacteria and Lactobacilli spp. are significantly increased at all intervals (7, 14, 28 and 40 days post supplementation) in the supplemented group compared with non-supplemented group with P< 0.001. On the other hand, there is a significant decrease in the count of E. coli and C. perfringens specially at 40 days post supplementation from (5.8 log 10 cfu ±0.06) to (3.1 log 10 cfu ±0.07) and from (4.8 log 10 cfu ±0.09) to (2.3 log 10 cfu ±0.09) respectively with P< 0.001. B. subtilis proved to be tolerant to the simulated gastric and intestinal juice for one hour (100% tolerance). However, after two hours, the tolerance to the gastric juice decreased to 70%, with no effect of the artificial intestinal juice on the viability of the organism. In vitro, the antimicrobial effect of B. subtilis on C. perfringens type A by well diffusion method showed an inhibition zone of 10 mm. Results of in vitro effect of B. subtilis on C. perfringens count showed that at 103 dilutions of C. perfringens, its count was reduced 2.1×105 to 3×104 after adding B. subtilis, while at dilution of 102, the count was reduced from 2.4×105 to 7×103cfu/ml after adding B. subtilis. It could be concluded that B. subtilis can be used as a probiotic in rabbits ration due to its ability to increase the commensal microflora count beside its antibacterial effect against some opportunistic pathogens.
format	article
author	Eman, F. Farag G. A. Abdel-Alim
author_facet	Eman, F. Farag G. A. Abdel-Alim
author_sort	Eman, F. Farag
title	The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
title_short	The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
title_full	The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
title_fullStr	The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
title_full_unstemmed	The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci
title_sort	effect of bacillus subtilis on the bacterial content in rabbits caeci
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.117995 https://doaj.org/article/6b5933e9b0d548c38d852c8a7bac8e05
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The Effect Of Bacillus Subtilis On The Bacterial Content In Rabbits Caeci

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