Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Abstract Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sarah Janati Idrissi, Daniel Le Bourhis, Antoine Lefevre, Patrick Emond, Laurene Le Berre, Olivier Desnoës, Thierry Joly, Samuel Buff, Virginie Maillard, Laurent Schibler, Pascal Salvetti, Sebastien Elis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6bb48b63a47e4f838eab9e1fcf3ae380
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6bb48b63a47e4f838eab9e1fcf3ae380
record_format	dspace
spelling	oai:doaj.org-article:6bb48b63a47e4f838eab9e1fcf3ae3802021-12-02T17:50:41ZLipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process10.1038/s41598-021-90870-82045-2322https://doaj.org/article/6bb48b63a47e4f838eab9e1fcf3ae3802021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90870-8https://doaj.org/toc/2045-2322Abstract Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.Sarah Janati IdrissiDaniel Le BourhisAntoine LefevrePatrick EmondLaurene Le BerreOlivier DesnoësThierry JolySamuel BuffVirginie MaillardLaurent SchiblerPascal SalvettiSebastien ElisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah Janati Idrissi Daniel Le Bourhis Antoine Lefevre Patrick Emond Laurene Le Berre Olivier Desnoës Thierry Joly Samuel Buff Virginie Maillard Laurent Schibler Pascal Salvetti Sebastien Elis Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
description	Abstract Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.
format	article
author	Sarah Janati Idrissi Daniel Le Bourhis Antoine Lefevre Patrick Emond Laurene Le Berre Olivier Desnoës Thierry Joly Samuel Buff Virginie Maillard Laurent Schibler Pascal Salvetti Sebastien Elis
author_facet	Sarah Janati Idrissi Daniel Le Bourhis Antoine Lefevre Patrick Emond Laurene Le Berre Olivier Desnoës Thierry Joly Samuel Buff Virginie Maillard Laurent Schibler Pascal Salvetti Sebastien Elis
author_sort	Sarah Janati Idrissi
title	Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
title_short	Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
title_full	Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
title_fullStr	Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
title_full_unstemmed	Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
title_sort	lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6bb48b63a47e4f838eab9e1fcf3ae380
work_keys_str_mv	AT sarahjanatiidrissi lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT daniellebourhis lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT antoinelefevre lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT patrickemond lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT laureneleberre lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT olivierdesnoes lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT thierryjoly lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT samuelbuff lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT virginiemaillard lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT laurentschibler lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT pascalsalvetti lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess AT sebastienelis lipidprofileofbovinegrade1blastocystsproducedeitherinvivoorinvitrobeforeandafterslowfreezingprocess
_version_	1718379290036273152

Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Ejemplares similares