Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

ABSTRACT Neutrophils are essential cells of host innate immunity. Although the role of neutrophils in defense against bacterial and fungal infections is well characterized, there is a relative paucity of information about their role against viral infections. Influenza A virus (IAV) infection can be...

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Autores principales:	Natalia Malachowa, Brett Freedman, Daniel E. Sturdevant, Scott D. Kobayashi, Vinod Nair, Friederike Feldmann, Tregei Starr, Olivia Steele-Mortimer, John C. Kash, Jeffery K. Taubenberger, Heinz Feldmann, Frank R. DeLeo
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	H1N1 Staphylococcus aureus immune response influenza A virus neutrophils Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6be56654bd414c9aa10bfe2cf1b87653
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spelling	oai:doaj.org-article:6be56654bd414c9aa10bfe2cf1b876532021-11-15T15:22:01ZDifferential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils10.1128/mSphereDirect.00567-172379-5042https://doaj.org/article/6be56654bd414c9aa10bfe2cf1b876532018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphereDirect.00567-17https://doaj.org/toc/2379-5042ABSTRACT Neutrophils are essential cells of host innate immunity. Although the role of neutrophils in defense against bacterial and fungal infections is well characterized, there is a relative paucity of information about their role against viral infections. Influenza A virus (IAV) infection can be associated with secondary bacterial coinfection, and it has long been posited that the ability of IAV to alter normal neutrophil function predisposes individuals to secondary bacterial infections. To better understand this phenomenon, we evaluated the interaction of pandemic or seasonal H1N1 IAV with human neutrophils isolated from healthy persons. These viruses were ingested by human neutrophils and elicited changes in neutrophil gene expression that are consistent with an interferon-mediated immune response. The viability of neutrophils following coculture with either pandemic or seasonal H1N1 IAV was similar for up to 18 h of culture. Notably, neutrophil exposure to seasonal (but not pandemic) IAV primed these leukocytes for enhanced functions, including production of reactive oxygen species and bactericidal activity. Taken together, our results are at variance with the universal idea that IAV impairs neutrophil function directly to predispose individuals to secondary bacterial infections. Rather, we suggest that some strains of IAV prime neutrophils for enhanced bacterial clearance. IMPORTANCE A long-standing notion is that IAV inhibits normal neutrophil function and thereby predisposes individuals to secondary bacterial infections. Here we report that seasonal H1N1 IAV primes human neutrophils for enhanced killing of Staphylococcus aureus. Moreover, we provide a comprehensive view of the changes in neutrophil gene expression during interaction with seasonal or pandemic IAV and report how these changes relate to functions such as bactericidal activity. This study expands our knowledge of IAV interactions with human neutrophils.Natalia MalachowaBrett FreedmanDaniel E. SturdevantScott D. KobayashiVinod NairFriederike FeldmannTregei StarrOlivia Steele-MortimerJohn C. KashJeffery K. TaubenbergerHeinz FeldmannFrank R. DeLeoAmerican Society for MicrobiologyarticleH1N1Staphylococcus aureusimmune responseinfluenza A virusneutrophilsMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	H1N1 Staphylococcus aureus immune response influenza A virus neutrophils Microbiology QR1-502
spellingShingle	H1N1 Staphylococcus aureus immune response influenza A virus neutrophils Microbiology QR1-502 Natalia Malachowa Brett Freedman Daniel E. Sturdevant Scott D. Kobayashi Vinod Nair Friederike Feldmann Tregei Starr Olivia Steele-Mortimer John C. Kash Jeffery K. Taubenberger Heinz Feldmann Frank R. DeLeo Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
description	ABSTRACT Neutrophils are essential cells of host innate immunity. Although the role of neutrophils in defense against bacterial and fungal infections is well characterized, there is a relative paucity of information about their role against viral infections. Influenza A virus (IAV) infection can be associated with secondary bacterial coinfection, and it has long been posited that the ability of IAV to alter normal neutrophil function predisposes individuals to secondary bacterial infections. To better understand this phenomenon, we evaluated the interaction of pandemic or seasonal H1N1 IAV with human neutrophils isolated from healthy persons. These viruses were ingested by human neutrophils and elicited changes in neutrophil gene expression that are consistent with an interferon-mediated immune response. The viability of neutrophils following coculture with either pandemic or seasonal H1N1 IAV was similar for up to 18 h of culture. Notably, neutrophil exposure to seasonal (but not pandemic) IAV primed these leukocytes for enhanced functions, including production of reactive oxygen species and bactericidal activity. Taken together, our results are at variance with the universal idea that IAV impairs neutrophil function directly to predispose individuals to secondary bacterial infections. Rather, we suggest that some strains of IAV prime neutrophils for enhanced bacterial clearance. IMPORTANCE A long-standing notion is that IAV inhibits normal neutrophil function and thereby predisposes individuals to secondary bacterial infections. Here we report that seasonal H1N1 IAV primes human neutrophils for enhanced killing of Staphylococcus aureus. Moreover, we provide a comprehensive view of the changes in neutrophil gene expression during interaction with seasonal or pandemic IAV and report how these changes relate to functions such as bactericidal activity. This study expands our knowledge of IAV interactions with human neutrophils.
format	article
author	Natalia Malachowa Brett Freedman Daniel E. Sturdevant Scott D. Kobayashi Vinod Nair Friederike Feldmann Tregei Starr Olivia Steele-Mortimer John C. Kash Jeffery K. Taubenberger Heinz Feldmann Frank R. DeLeo
author_facet	Natalia Malachowa Brett Freedman Daniel E. Sturdevant Scott D. Kobayashi Vinod Nair Friederike Feldmann Tregei Starr Olivia Steele-Mortimer John C. Kash Jeffery K. Taubenberger Heinz Feldmann Frank R. DeLeo
author_sort	Natalia Malachowa
title	Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
title_short	Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
title_full	Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
title_fullStr	Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
title_full_unstemmed	Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils
title_sort	differential ability of pandemic and seasonal h1n1 influenza a viruses to alter the function of human neutrophils
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/6be56654bd414c9aa10bfe2cf1b87653
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Differential Ability of Pandemic and Seasonal H1N1 Influenza A Viruses To Alter the Function of Human Neutrophils

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