Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis

Multiple sclerosis is chronic demyelinating disease of the central nervous system with autoimmune inflammation and accretive neurodegeneration. One of the characteristics of autoimmune inflammation in multiple sclerosis is a polyspecific intrathecal humoral immune response against neurotropic viruse...

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Autores principales:	A. N. Moshnikova, V. K. Maksimchuk, S. V. Lapin, V. D. Nazarov, E. A. Surkova, S. A. Novikov, G. S. Makshakov, I. Yu. Krutetskaya, V. S. Krasnov, Ya. B. Kushnir, N. A. Neofidov, N. A. Totolian, A. A. Skoromets, E. P. Evdoshenko, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	multiple sclerosis mrz-reaction intrathecal synthesis of immunoglobulins free light chains of immunoglobulins oligoclonal igg antibody indices Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6c3d14a17f2a4c6ba344cb2e4b7914c4
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spelling	oai:doaj.org-article:6c3d14a17f2a4c6ba344cb2e4b7914c42021-11-22T07:09:53ZDiagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis2220-76192313-739810.15789/2220-7619-2019-5-6-703-712https://doaj.org/article/6c3d14a17f2a4c6ba344cb2e4b7914c42020-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/754https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Multiple sclerosis is chronic demyelinating disease of the central nervous system with autoimmune inflammation and accretive neurodegeneration. One of the characteristics of autoimmune inflammation in multiple sclerosis is a polyspecific intrathecal humoral immune response against neurotropic viruses (Measles, Rubella and varicella Zoster) called MRZ-reaction. This immune response is based on polyclonal activation of mature B lymphocytes in the central nervous system and intrathecal synthesis of antibodies to anamnestic antigens unrelated to viral replication in the central nervous system as well as serum antibody release. Immunoglobulins produced against neurotropic viruses are an integral part of the oligoclonal antibody pool in the cerebrospinal fluid. Because immunoglobulins can penetrate the blood brain barrier, not only serum and cerebrospinal fluid specific antibody indices are calculated, but also blood-brain barrier antibody permeability (Qalbumin, QIgG) are taken into account to assess their intrathecal synthesis. The aim of the study was to assess the informative value of the intrathecal antibodies against neurotropic viruses (MRZ-reaction) in multiple sclerosis. There were enrolled 60 patients divided into 2 groups: group 1 — 35 patients diagnosed with multiple sclerosis, group 2 — 25 patients with inflammatory and non-inflammatory disоrders of the central nervous system. Paired cerebrospinal fluid and serum samples were collected from all patients to measure level of oligoclonal IgG, immunoglobulin free kappa and lambda light chains, IgG index and specific antibodies indices, followed by assessing magnitude of MRZ-reaction. We found that the MRZ-reaction was the most specific test to diagnose multiple sclerosis. Intrathecally produced antibodies against neurotropic viruses were detected in 3 of 35 multiple sclerosis patients with lacking oligoclonal IgG antibodies. In addition, a relationship between MRZ-reaction and degree of EDSS disability was found in MS patients: peak EDSS score was reported in patients with intrathecally synthesized antibodies against 3 viral agents, whereas the minimum EDSS score — among MRZ-negative patients. Thus, assessing MRZ-reaction seems rational for confirming MS diagnosis in case of other negative laboratory tests (oligoclonal immunoglobulins and free kappa/lambda light chains in cerebrospinal fluid) allowing to improves diagnostic accuracy and evaluation of MS severity.A. N. MoshnikovaV. K. MaksimchukS. V. LapinV. D. NazarovE. A. SurkovaS. A. NovikovG. S. MakshakovI. Yu. KrutetskayaV. S. KrasnovYa. B. KushnirN. A. NeofidovN. A. TotolianA. A. SkorometsE. P. EvdoshenkoAreg A. TotolianSankt-Peterburg : NIIÈM imeni Pasteraarticlemultiple sclerosismrz-reactionintrathecal synthesis of immunoglobulinsfree light chains of immunoglobulinsoligoclonal iggantibody indicesInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 5-6, Pp 703-712 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	multiple sclerosis mrz-reaction intrathecal synthesis of immunoglobulins free light chains of immunoglobulins oligoclonal igg antibody indices Infectious and parasitic diseases RC109-216
spellingShingle	multiple sclerosis mrz-reaction intrathecal synthesis of immunoglobulins free light chains of immunoglobulins oligoclonal igg antibody indices Infectious and parasitic diseases RC109-216 A. N. Moshnikova V. K. Maksimchuk S. V. Lapin V. D. Nazarov E. A. Surkova S. A. Novikov G. S. Makshakov I. Yu. Krutetskaya V. S. Krasnov Ya. B. Kushnir N. A. Neofidov N. A. Totolian A. A. Skoromets E. P. Evdoshenko Areg A. Totolian Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
description	Multiple sclerosis is chronic demyelinating disease of the central nervous system with autoimmune inflammation and accretive neurodegeneration. One of the characteristics of autoimmune inflammation in multiple sclerosis is a polyspecific intrathecal humoral immune response against neurotropic viruses (Measles, Rubella and varicella Zoster) called MRZ-reaction. This immune response is based on polyclonal activation of mature B lymphocytes in the central nervous system and intrathecal synthesis of antibodies to anamnestic antigens unrelated to viral replication in the central nervous system as well as serum antibody release. Immunoglobulins produced against neurotropic viruses are an integral part of the oligoclonal antibody pool in the cerebrospinal fluid. Because immunoglobulins can penetrate the blood brain barrier, not only serum and cerebrospinal fluid specific antibody indices are calculated, but also blood-brain barrier antibody permeability (Qalbumin, QIgG) are taken into account to assess their intrathecal synthesis. The aim of the study was to assess the informative value of the intrathecal antibodies against neurotropic viruses (MRZ-reaction) in multiple sclerosis. There were enrolled 60 patients divided into 2 groups: group 1 — 35 patients diagnosed with multiple sclerosis, group 2 — 25 patients with inflammatory and non-inflammatory disоrders of the central nervous system. Paired cerebrospinal fluid and serum samples were collected from all patients to measure level of oligoclonal IgG, immunoglobulin free kappa and lambda light chains, IgG index and specific antibodies indices, followed by assessing magnitude of MRZ-reaction. We found that the MRZ-reaction was the most specific test to diagnose multiple sclerosis. Intrathecally produced antibodies against neurotropic viruses were detected in 3 of 35 multiple sclerosis patients with lacking oligoclonal IgG antibodies. In addition, a relationship between MRZ-reaction and degree of EDSS disability was found in MS patients: peak EDSS score was reported in patients with intrathecally synthesized antibodies against 3 viral agents, whereas the minimum EDSS score — among MRZ-negative patients. Thus, assessing MRZ-reaction seems rational for confirming MS diagnosis in case of other negative laboratory tests (oligoclonal immunoglobulins and free kappa/lambda light chains in cerebrospinal fluid) allowing to improves diagnostic accuracy and evaluation of MS severity.
format	article
author	A. N. Moshnikova V. K. Maksimchuk S. V. Lapin V. D. Nazarov E. A. Surkova S. A. Novikov G. S. Makshakov I. Yu. Krutetskaya V. S. Krasnov Ya. B. Kushnir N. A. Neofidov N. A. Totolian A. A. Skoromets E. P. Evdoshenko Areg A. Totolian
author_facet	A. N. Moshnikova V. K. Maksimchuk S. V. Lapin V. D. Nazarov E. A. Surkova S. A. Novikov G. S. Makshakov I. Yu. Krutetskaya V. S. Krasnov Ya. B. Kushnir N. A. Neofidov N. A. Totolian A. A. Skoromets E. P. Evdoshenko Areg A. Totolian
author_sort	A. N. Moshnikova
title	Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
title_short	Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
title_full	Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
title_fullStr	Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
title_full_unstemmed	Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis
title_sort	diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (mrz-reaction) in diagnosis of multiple sclerosis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/6c3d14a17f2a4c6ba344cb2e4b7914c4
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Diagnostic significance of intrathecally synthesized immunoglobulins against neurotropic viruses (MRZ-reaction) in diagnosis of multiple sclerosis

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