DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. Here, the authors use a DNA origami-based force clamp to investigate the assembly dynamics of human initiation complexes in the RNAP II and RNAP III systems a...

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Autores principales: Kevin Kramm, Tim Schröder, Jerome Gouge, Andrés Manuel Vera, Kapil Gupta, Florian B. Heiss, Tim Liedl, Christoph Engel, Imre Berger, Alessandro Vannini, Philip Tinnefeld, Dina Grohmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/6c52a4bb73744eafa2b7a2b2daca7321
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