Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder

Methamphetamine use disorder (MUD) is a major public health problem worldwide with limited effective treatment options. Previous studies have reported methamphetamine-associated alterations in gut microbiota. A potential role of gut microbiota in regulating methamphetamine-induced brain dysfunction...

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Autores principales: Di Deng, Hang Su, Yuehong Song, Tianzhen Chen, Qianqian Sun, Haifeng Jiang, Min Zhao
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Publicado: Frontiers Media S.A. 2021
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spelling oai:doaj.org-article:6cff3401db2d4bab82bc21b076f21fa92021-11-18T09:47:11ZAltered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder2235-298810.3389/fcimb.2021.783917https://doaj.org/article/6cff3401db2d4bab82bc21b076f21fa92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.783917/fullhttps://doaj.org/toc/2235-2988Methamphetamine use disorder (MUD) is a major public health problem worldwide with limited effective treatment options. Previous studies have reported methamphetamine-associated alterations in gut microbiota. A potential role of gut microbiota in regulating methamphetamine-induced brain dysfunction through interactions with the host immune system has been proposed, but evidence for this hypothesis is limited. The present study aimed to investigate the alterations in the fecal microbiota and explore its relationship with systemic inflammation in MUD. Fecal samples were obtained from 26 male subjects with MUD and 17 sex- and age- matched healthy controls. Fecal microbial profiles were analyzed by 16S rRNA sequencing. Plasma inflammatory markers were measured using enzyme-linked immunosorbent assay. Associations between fecal microbiota, systemic inflammatory markers and clinical characteristics were examined by Spearman partial correlation analysis while controlling for possible confounders. Compared with healthy controls, individuals with MUD showed no difference in fecal microbial diversity, but exhibited differences in the relative abundance of several microbial taxa. At the genus level, a higher abundance of Collinsella, Odoribacter and Megasphaera and lower levels of Faecalibacterium, Blautia, Dorea and Streptococcus were detected in subjects with MUD. More importantly, altered fecal microbiota was found to be correlated with plasma levels of CRP, IL-2, IL-6 and IL-10. The order Lactobacillales, exhibiting lower abundance in participants with MUD, was positively related to the duration of methamphetamine abstinence and the plasma level of anti-inflammatory cytokine IL-10. This study is the first to provide evidence for a link between altered fecal microbiota and systemic inflammation in MUD. Further elucidation of interactions between gut microbiota and the host immune system may be beneficial for the development of novel therapeutic approaches for MUD.Di DengHang SuYuehong SongTianzhen ChenQianqian SunHaifeng JiangMin ZhaoMin ZhaoMin ZhaoFrontiers Media S.A.articlemethamphetamine use disordergut microbiota16S rRNA sequencesystemic inflammationgut-immune-brainMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic methamphetamine use disorder
gut microbiota
16S rRNA sequence
systemic inflammation
gut-immune-brain
Microbiology
QR1-502
spellingShingle methamphetamine use disorder
gut microbiota
16S rRNA sequence
systemic inflammation
gut-immune-brain
Microbiology
QR1-502
Di Deng
Hang Su
Yuehong Song
Tianzhen Chen
Qianqian Sun
Haifeng Jiang
Min Zhao
Min Zhao
Min Zhao
Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
description Methamphetamine use disorder (MUD) is a major public health problem worldwide with limited effective treatment options. Previous studies have reported methamphetamine-associated alterations in gut microbiota. A potential role of gut microbiota in regulating methamphetamine-induced brain dysfunction through interactions with the host immune system has been proposed, but evidence for this hypothesis is limited. The present study aimed to investigate the alterations in the fecal microbiota and explore its relationship with systemic inflammation in MUD. Fecal samples were obtained from 26 male subjects with MUD and 17 sex- and age- matched healthy controls. Fecal microbial profiles were analyzed by 16S rRNA sequencing. Plasma inflammatory markers were measured using enzyme-linked immunosorbent assay. Associations between fecal microbiota, systemic inflammatory markers and clinical characteristics were examined by Spearman partial correlation analysis while controlling for possible confounders. Compared with healthy controls, individuals with MUD showed no difference in fecal microbial diversity, but exhibited differences in the relative abundance of several microbial taxa. At the genus level, a higher abundance of Collinsella, Odoribacter and Megasphaera and lower levels of Faecalibacterium, Blautia, Dorea and Streptococcus were detected in subjects with MUD. More importantly, altered fecal microbiota was found to be correlated with plasma levels of CRP, IL-2, IL-6 and IL-10. The order Lactobacillales, exhibiting lower abundance in participants with MUD, was positively related to the duration of methamphetamine abstinence and the plasma level of anti-inflammatory cytokine IL-10. This study is the first to provide evidence for a link between altered fecal microbiota and systemic inflammation in MUD. Further elucidation of interactions between gut microbiota and the host immune system may be beneficial for the development of novel therapeutic approaches for MUD.
format article
author Di Deng
Hang Su
Yuehong Song
Tianzhen Chen
Qianqian Sun
Haifeng Jiang
Min Zhao
Min Zhao
Min Zhao
author_facet Di Deng
Hang Su
Yuehong Song
Tianzhen Chen
Qianqian Sun
Haifeng Jiang
Min Zhao
Min Zhao
Min Zhao
author_sort Di Deng
title Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
title_short Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
title_full Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
title_fullStr Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
title_full_unstemmed Altered Fecal Microbiota Correlated With Systemic Inflammation in Male Subjects With Methamphetamine Use Disorder
title_sort altered fecal microbiota correlated with systemic inflammation in male subjects with methamphetamine use disorder
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/6cff3401db2d4bab82bc21b076f21fa9
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