Cell Cycle Regulation by Alternative Polyadenylation of CCND1

Abstract Global shortening of 3′UTRs by alternative polyadenylation (APA) has been observed in cancer cells. However, the role of APA in cancer remains unknown. CCND1 is a proto-oncogene that regulates progression through the G1-S phase of the cell cycle; moreover, it has been observed to be switchi...

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Autores principales:	Qiong Wang, Guopei He, Mengmeng Hou, Liutao Chen, Shangwu Chen, Anlong Xu, Yonggui Fu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d2ecd47812c4c7a822f6dc174b416a0
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spelling	oai:doaj.org-article:6d2ecd47812c4c7a822f6dc174b416a02021-12-02T11:40:53ZCell Cycle Regulation by Alternative Polyadenylation of CCND110.1038/s41598-018-25141-02045-2322https://doaj.org/article/6d2ecd47812c4c7a822f6dc174b416a02018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25141-0https://doaj.org/toc/2045-2322Abstract Global shortening of 3′UTRs by alternative polyadenylation (APA) has been observed in cancer cells. However, the role of APA in cancer remains unknown. CCND1 is a proto-oncogene that regulates progression through the G1-S phase of the cell cycle; moreover, it has been observed to be switching to proximal APA sites in cancer cells. To investigate the biological function of the APA of CCND1, we edited the weak poly(A) signal (PAS) of the proximal APA site to a canonical PAS using the CRISPR/Cas9 method, which can force the cells to use a proximal APA site. Cell cycle profiling and proliferation assays revealed that the proximal APA sites of CCND1 accelerated the cell cycle and promoted cell proliferation, but UTR-APA and CR-APA act via different molecular mechanisms. These results indicate that PAS editing with CRISPR/Cas9 provides a good method by which to study the biological function of APA.Qiong WangGuopei HeMengmeng HouLiutao ChenShangwu ChenAnlong XuYonggui FuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiong Wang Guopei He Mengmeng Hou Liutao Chen Shangwu Chen Anlong Xu Yonggui Fu Cell Cycle Regulation by Alternative Polyadenylation of CCND1
description	Abstract Global shortening of 3′UTRs by alternative polyadenylation (APA) has been observed in cancer cells. However, the role of APA in cancer remains unknown. CCND1 is a proto-oncogene that regulates progression through the G1-S phase of the cell cycle; moreover, it has been observed to be switching to proximal APA sites in cancer cells. To investigate the biological function of the APA of CCND1, we edited the weak poly(A) signal (PAS) of the proximal APA site to a canonical PAS using the CRISPR/Cas9 method, which can force the cells to use a proximal APA site. Cell cycle profiling and proliferation assays revealed that the proximal APA sites of CCND1 accelerated the cell cycle and promoted cell proliferation, but UTR-APA and CR-APA act via different molecular mechanisms. These results indicate that PAS editing with CRISPR/Cas9 provides a good method by which to study the biological function of APA.
format	article
author	Qiong Wang Guopei He Mengmeng Hou Liutao Chen Shangwu Chen Anlong Xu Yonggui Fu
author_facet	Qiong Wang Guopei He Mengmeng Hou Liutao Chen Shangwu Chen Anlong Xu Yonggui Fu
author_sort	Qiong Wang
title	Cell Cycle Regulation by Alternative Polyadenylation of CCND1
title_short	Cell Cycle Regulation by Alternative Polyadenylation of CCND1
title_full	Cell Cycle Regulation by Alternative Polyadenylation of CCND1
title_fullStr	Cell Cycle Regulation by Alternative Polyadenylation of CCND1
title_full_unstemmed	Cell Cycle Regulation by Alternative Polyadenylation of CCND1
title_sort	cell cycle regulation by alternative polyadenylation of ccnd1
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6d2ecd47812c4c7a822f6dc174b416a0
work_keys_str_mv	AT qiongwang cellcycleregulationbyalternativepolyadenylationofccnd1 AT guopeihe cellcycleregulationbyalternativepolyadenylationofccnd1 AT mengmenghou cellcycleregulationbyalternativepolyadenylationofccnd1 AT liutaochen cellcycleregulationbyalternativepolyadenylationofccnd1 AT shangwuchen cellcycleregulationbyalternativepolyadenylationofccnd1 AT anlongxu cellcycleregulationbyalternativepolyadenylationofccnd1 AT yongguifu cellcycleregulationbyalternativepolyadenylationofccnd1
_version_	1718395521575419904

Cell Cycle Regulation by Alternative Polyadenylation of CCND1

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