Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei

The structure and dynamics of large proteins and complexes can be studied by methyl-NMR but resonance assignment is still challenging. Here, the authors present a NMR method that leverages optimal control pulse design to unambiguously distinguish between Leu and Val using a simple 2D HMQC experiment...

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Autores principales:	Soumya P. Behera, Abhinav Dubey, Wan-Na Chen, Viviane S. De Paula, Meng Zhang, Nikolaos G. Sgourakis, Wolfgang Bermel, Gerhard Wagner, Paul W. Coote, Haribabu Arthanari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6d3ce66bd2fb4ffa8c649ac9c398510f
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spelling	oai:doaj.org-article:6d3ce66bd2fb4ffa8c649ac9c398510f2021-12-02T17:31:55ZNearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei10.1038/s41467-020-19325-42041-1723https://doaj.org/article/6d3ce66bd2fb4ffa8c649ac9c398510f2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19325-4https://doaj.org/toc/2041-1723The structure and dynamics of large proteins and complexes can be studied by methyl-NMR but resonance assignment is still challenging. Here, the authors present a NMR method that leverages optimal control pulse design to unambiguously distinguish between Leu and Val using a simple 2D HMQC experiment and they apply it to several proteins including Cas9, interleukin, and human translation initiation factor eIF4a.Soumya P. BeheraAbhinav DubeyWan-Na ChenViviane S. De PaulaMeng ZhangNikolaos G. SgourakisWolfgang BermelGerhard WagnerPaul W. CooteHaribabu ArthanariNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Soumya P. Behera Abhinav Dubey Wan-Na Chen Viviane S. De Paula Meng Zhang Nikolaos G. Sgourakis Wolfgang Bermel Gerhard Wagner Paul W. Coote Haribabu Arthanari Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
description	The structure and dynamics of large proteins and complexes can be studied by methyl-NMR but resonance assignment is still challenging. Here, the authors present a NMR method that leverages optimal control pulse design to unambiguously distinguish between Leu and Val using a simple 2D HMQC experiment and they apply it to several proteins including Cas9, interleukin, and human translation initiation factor eIF4a.
format	article
author	Soumya P. Behera Abhinav Dubey Wan-Na Chen Viviane S. De Paula Meng Zhang Nikolaos G. Sgourakis Wolfgang Bermel Gerhard Wagner Paul W. Coote Haribabu Arthanari
author_facet	Soumya P. Behera Abhinav Dubey Wan-Na Chen Viviane S. De Paula Meng Zhang Nikolaos G. Sgourakis Wolfgang Bermel Gerhard Wagner Paul W. Coote Haribabu Arthanari
author_sort	Soumya P. Behera
title	Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
title_short	Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
title_full	Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
title_fullStr	Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
title_full_unstemmed	Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
title_sort	nearest-neighbor nmr spectroscopy: categorizing spectral peaks by their adjacent nuclei
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6d3ce66bd2fb4ffa8c649ac9c398510f
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Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei

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