Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Abstract Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from...

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Bibliographic Details
Main Authors: David Falck, Markus Haberger, Rosina Plomp, Michaela Hook, Patrick Bulau, Manfred Wuhrer, Dietmar Reusch
Format: article
Language:EN
Published: Nature Portfolio 2017
Subjects:
Medicine
R
Science
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Online Access:https://doaj.org/article/6d50a3c6a01c4ababda8c549c01c7c0a
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