Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria

A total of 15 strains of lactic acid bacteria (LAB) were isolated from the broiler chicken’s gastrointestinal tract. All isolates were phenotypical and genetically identified. Among these isolates, only six were biochemical (API 50 CHL and ABIS soft) and genetically (16S rRNA sequencing) confirmed a...

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Autores principales:	Mihaela Dumitru, Dan Cristian Vodnar, Simon Elemer, Georgeta Ciurescu, Mihaela Habeanu, Ionut Sorescu, Sergiu Emil Georgescu, Andreea Dudu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lactobacillus</i> probiotic spray drying viability broiler Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6d933e3e871947428fb267186f2fa996
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spelling	oai:doaj.org-article:6d933e3e871947428fb267186f2fa9962021-11-11T14:59:31ZEvaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria10.3390/app112198672076-3417https://doaj.org/article/6d933e3e871947428fb267186f2fa9962021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9867https://doaj.org/toc/2076-3417A total of 15 strains of lactic acid bacteria (LAB) were isolated from the broiler chicken’s gastrointestinal tract. All isolates were phenotypical and genetically identified. Among these isolates, only six were biochemical (API 50 CHL and ABIS soft) and genetically (16S rRNA sequencing) confirmed as <i>Lactobacillus acidophilus</i>, <i>Limosilactobacillus fermentum</i>, <i>Levilactobacillus brevis</i>, and <i>Ligilactobacillus salivarius</i>. Probiotic properties, including tolerance to pH (pH 2.0 and 3.0), bile salts (0.3% oxgall), hemolysis activity, and antibiotic susceptibility, were evaluated. Three isolates of the latter isolates showed high resistance at low pH values (73.74% to 98.20%) and bile salt concentrations (77.89% to 99.49%). The antibiotic test presented 100% resistance of LAB to gentamicin, lincomycin, enrofloxacin, and streptomycin lower than the 0.5 mm inhibition zone diameter. Selected strains (<i>L. acidophilus</i> IBNA 64, <i>L. salivarius</i> IBNA 33, and <i>L. salivarius</i> IBNA 41) were exposed to the spray-drying process based on observable probiotic potential. A maltodextrin-glucose solution was used as a thermoprotectant. After spray drying, a reduction in strain viability was noted (10<sup>8</sup> to 10<sup>4</sup> CFU/g). In conclusion, only <i>L. salivarius</i> (IBNA 33 and IBNA 41) could be used as a possible probiotic, and further studies are needed to ensure their safe application in the animal nutrition field with beneficial effects for improving performance and pathogen microorganism control from intestines equilibrating the microbiota composition.Mihaela DumitruDan Cristian VodnarSimon ElemerGeorgeta CiurescuMihaela HabeanuIonut SorescuSergiu Emil GeorgescuAndreea DuduMDPI AGarticle<i>Lactobacillus</i>probioticspray dryingviabilitybroilerTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9867, p 9867 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lactobacillus</i> probiotic spray drying viability broiler Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	<i>Lactobacillus</i> probiotic spray drying viability broiler Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Mihaela Dumitru Dan Cristian Vodnar Simon Elemer Georgeta Ciurescu Mihaela Habeanu Ionut Sorescu Sergiu Emil Georgescu Andreea Dudu Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
description	A total of 15 strains of lactic acid bacteria (LAB) were isolated from the broiler chicken’s gastrointestinal tract. All isolates were phenotypical and genetically identified. Among these isolates, only six were biochemical (API 50 CHL and ABIS soft) and genetically (16S rRNA sequencing) confirmed as <i>Lactobacillus acidophilus</i>, <i>Limosilactobacillus fermentum</i>, <i>Levilactobacillus brevis</i>, and <i>Ligilactobacillus salivarius</i>. Probiotic properties, including tolerance to pH (pH 2.0 and 3.0), bile salts (0.3% oxgall), hemolysis activity, and antibiotic susceptibility, were evaluated. Three isolates of the latter isolates showed high resistance at low pH values (73.74% to 98.20%) and bile salt concentrations (77.89% to 99.49%). The antibiotic test presented 100% resistance of LAB to gentamicin, lincomycin, enrofloxacin, and streptomycin lower than the 0.5 mm inhibition zone diameter. Selected strains (<i>L. acidophilus</i> IBNA 64, <i>L. salivarius</i> IBNA 33, and <i>L. salivarius</i> IBNA 41) were exposed to the spray-drying process based on observable probiotic potential. A maltodextrin-glucose solution was used as a thermoprotectant. After spray drying, a reduction in strain viability was noted (10<sup>8</sup> to 10<sup>4</sup> CFU/g). In conclusion, only <i>L. salivarius</i> (IBNA 33 and IBNA 41) could be used as a possible probiotic, and further studies are needed to ensure their safe application in the animal nutrition field with beneficial effects for improving performance and pathogen microorganism control from intestines equilibrating the microbiota composition.
format	article
author	Mihaela Dumitru Dan Cristian Vodnar Simon Elemer Georgeta Ciurescu Mihaela Habeanu Ionut Sorescu Sergiu Emil Georgescu Andreea Dudu
author_facet	Mihaela Dumitru Dan Cristian Vodnar Simon Elemer Georgeta Ciurescu Mihaela Habeanu Ionut Sorescu Sergiu Emil Georgescu Andreea Dudu
author_sort	Mihaela Dumitru
title	Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
title_short	Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
title_full	Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
title_fullStr	Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
title_full_unstemmed	Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria
title_sort	evaluation of non-encapsulated and microencapsulated lactic acid bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6d933e3e871947428fb267186f2fa996
work_keys_str_mv	AT mihaeladumitru evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT dancristianvodnar evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT simonelemer evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT georgetaciurescu evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT mihaelahabeanu evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT ionutsorescu evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT sergiuemilgeorgescu evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria AT andreeadudu evaluationofnonencapsulatedandmicroencapsulatedlacticacidbacteria
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Evaluation of Non-Encapsulated and Microencapsulated Lactic Acid Bacteria

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