Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Altering cellular responses to double-strand breaks in DNA could rebalance CRISPRediting outcomes. Here, the authors use a pooled CRISPR screen to identify inhibition of CDC7 as a strategy to improve HDR outcomes.

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Detalles Bibliográficos
Autores principales:	Beeke Wienert, David N. Nguyen, Alexis Guenther, Sharon J. Feng, Melissa N. Locke, Stacia K. Wyman, Jiyung Shin, Katelynn R. Kazane, Georgia L. Gregory, Matthew A. M. Carter, Francis Wright, Bruce R. Conklin, Alex Marson, Chris D. Richardson, Jacob E. Corn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6ea294e461964f21b3fa07be064041d4
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spelling	oai:doaj.org-article:6ea294e461964f21b3fa07be064041d42021-12-02T15:39:14ZTimed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair10.1038/s41467-020-15845-12041-1723https://doaj.org/article/6ea294e461964f21b3fa07be064041d42020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15845-1https://doaj.org/toc/2041-1723Altering cellular responses to double-strand breaks in DNA could rebalance CRISPRediting outcomes. Here, the authors use a pooled CRISPR screen to identify inhibition of CDC7 as a strategy to improve HDR outcomes.Beeke WienertDavid N. NguyenAlexis GuentherSharon J. FengMelissa N. LockeStacia K. WymanJiyung ShinKatelynn R. KazaneGeorgia L. GregoryMatthew A. M. CarterFrancis WrightBruce R. ConklinAlex MarsonChris D. RichardsonJacob E. CornNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Beeke Wienert David N. Nguyen Alexis Guenther Sharon J. Feng Melissa N. Locke Stacia K. Wyman Jiyung Shin Katelynn R. Kazane Georgia L. Gregory Matthew A. M. Carter Francis Wright Bruce R. Conklin Alex Marson Chris D. Richardson Jacob E. Corn Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
description	Altering cellular responses to double-strand breaks in DNA could rebalance CRISPRediting outcomes. Here, the authors use a pooled CRISPR screen to identify inhibition of CDC7 as a strategy to improve HDR outcomes.
format	article
author	Beeke Wienert David N. Nguyen Alexis Guenther Sharon J. Feng Melissa N. Locke Stacia K. Wyman Jiyung Shin Katelynn R. Kazane Georgia L. Gregory Matthew A. M. Carter Francis Wright Bruce R. Conklin Alex Marson Chris D. Richardson Jacob E. Corn
author_facet	Beeke Wienert David N. Nguyen Alexis Guenther Sharon J. Feng Melissa N. Locke Stacia K. Wyman Jiyung Shin Katelynn R. Kazane Georgia L. Gregory Matthew A. M. Carter Francis Wright Bruce R. Conklin Alex Marson Chris D. Richardson Jacob E. Corn
author_sort	Beeke Wienert
title	Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
title_short	Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
title_full	Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
title_fullStr	Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
title_full_unstemmed	Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
title_sort	timed inhibition of cdc7 increases crispr-cas9 mediated templated repair
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6ea294e461964f21b3fa07be064041d4
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Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

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