Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions
Mutations in human pluripotent stem cells (PSC) and whether any form during culture prior to use in a human clinical context are a concern. Here, the authors use hPSCs derived to cGMP standards and show they have low mutation rates after culture, noting this decreases on culturing in low (5%) oxygen...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/6f55e007701f4013b3a7b971436b8888 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:6f55e007701f4013b3a7b971436b8888 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:6f55e007701f4013b3a7b971436b88882021-12-02T14:42:12ZLow rates of mutation in clinical grade human pluripotent stem cells under different culture conditions10.1038/s41467-020-15271-32041-1723https://doaj.org/article/6f55e007701f4013b3a7b971436b88882020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15271-3https://doaj.org/toc/2041-1723Mutations in human pluripotent stem cells (PSC) and whether any form during culture prior to use in a human clinical context are a concern. Here, the authors use hPSCs derived to cGMP standards and show they have low mutation rates after culture, noting this decreases on culturing in low (5%) oxygen conditions.Oliver ThompsonFerdinand von MeyennZoe HewittJohn AlexanderAndrew WoodRichard WeightmanSian GregoryFelix KruegerSimon AndrewsIvana BarbaricPaul J. GokhaleHarry D. MooreWolf ReikMarta MiloSerena Nik-ZainalKosuke YusaPeter W. AndrewsNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Oliver Thompson Ferdinand von Meyenn Zoe Hewitt John Alexander Andrew Wood Richard Weightman Sian Gregory Felix Krueger Simon Andrews Ivana Barbaric Paul J. Gokhale Harry D. Moore Wolf Reik Marta Milo Serena Nik-Zainal Kosuke Yusa Peter W. Andrews Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| description |
Mutations in human pluripotent stem cells (PSC) and whether any form during culture prior to use in a human clinical context are a concern. Here, the authors use hPSCs derived to cGMP standards and show they have low mutation rates after culture, noting this decreases on culturing in low (5%) oxygen conditions. |
| format |
article |
| author |
Oliver Thompson Ferdinand von Meyenn Zoe Hewitt John Alexander Andrew Wood Richard Weightman Sian Gregory Felix Krueger Simon Andrews Ivana Barbaric Paul J. Gokhale Harry D. Moore Wolf Reik Marta Milo Serena Nik-Zainal Kosuke Yusa Peter W. Andrews |
| author_facet |
Oliver Thompson Ferdinand von Meyenn Zoe Hewitt John Alexander Andrew Wood Richard Weightman Sian Gregory Felix Krueger Simon Andrews Ivana Barbaric Paul J. Gokhale Harry D. Moore Wolf Reik Marta Milo Serena Nik-Zainal Kosuke Yusa Peter W. Andrews |
| author_sort |
Oliver Thompson |
| title |
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| title_short |
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| title_full |
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| title_fullStr |
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| title_full_unstemmed |
Low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| title_sort |
low rates of mutation in clinical grade human pluripotent stem cells under different culture conditions |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/6f55e007701f4013b3a7b971436b8888 |
| work_keys_str_mv |
AT oliverthompson lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT ferdinandvonmeyenn lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT zoehewitt lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT johnalexander lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT andrewwood lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT richardweightman lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT siangregory lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT felixkrueger lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT simonandrews lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT ivanabarbaric lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT pauljgokhale lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT harrydmoore lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT wolfreik lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT martamilo lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT serenanikzainal lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT kosukeyusa lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions AT peterwandrews lowratesofmutationinclinicalgradehumanpluripotentstemcellsunderdifferentcultureconditions |
| _version_ |
1718389756502474752 |