Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves

Besides aroma and taste, the color of dry tea leaves, tea infusion, and infused tea leaves is also an important index for tea quality. Shading can significantly increase the chlorophyll content of tea leaves, leading to enhanced tea leaf coloration. However, the underlying regulatory mechanism remai...

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Autores principales:	Jiaming Chen, Shuhua Wu, Fang Dong, Jianlong Li, Lanting Zeng, Jinchi Tang, Dachuan Gu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	shading light intensity chlorophyll accumulation Camellia sinensis transcription regulation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6fecfc118a8d448697e6b91e04948f8f
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spelling	oai:doaj.org-article:6fecfc118a8d448697e6b91e04948f8f2021-12-02T11:50:30ZMechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves1664-462X10.3389/fpls.2021.779819https://doaj.org/article/6fecfc118a8d448697e6b91e04948f8f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.779819/fullhttps://doaj.org/toc/1664-462XBesides aroma and taste, the color of dry tea leaves, tea infusion, and infused tea leaves is also an important index for tea quality. Shading can significantly increase the chlorophyll content of tea leaves, leading to enhanced tea leaf coloration. However, the underlying regulatory mechanism remains unclear. In this study, we revealed that the expressions of chlorophyll synthesis genes were significantly induced by shading, specially, the gene encoding protochlorophyllide oxidoreductase (CsPOR). Indoor control experiment showed that decreased light intensity could significantly induce the expression of CsPOR, and thus cause the increase of chlorophyll content. Subsequently, we explored the light signaling pathway transcription factors regulating chlorophyll synthesis, including CsPIFs and CsHY5. Through expression level and subcellular localization analysis, we found that CsPIF3-2, CsPIF7-1, and CsHY5 may be candidate transcriptional regulators. Transcriptional activation experiments proved that CsHY5 inhibits CsPORL-2 transcription. In summary, we concluded that shading might promote the expression of CsPORL-2 by inhibiting the expression of CsHY5, leading to high accumulation of chlorophyll in tea leaves. The results of this study provide insights into the mechanism regulating the improvements to tea plant quality caused by shading.Jiaming ChenJiaming ChenShuhua WuShuhua WuFang DongJianlong LiLanting ZengJinchi TangDachuan GuFrontiers Media S.A.articleshadinglight intensitychlorophyll accumulationCamellia sinensistranscription regulationPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	shading light intensity chlorophyll accumulation Camellia sinensis transcription regulation Plant culture SB1-1110
spellingShingle	shading light intensity chlorophyll accumulation Camellia sinensis transcription regulation Plant culture SB1-1110 Jiaming Chen Jiaming Chen Shuhua Wu Shuhua Wu Fang Dong Jianlong Li Lanting Zeng Jinchi Tang Dachuan Gu Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
description	Besides aroma and taste, the color of dry tea leaves, tea infusion, and infused tea leaves is also an important index for tea quality. Shading can significantly increase the chlorophyll content of tea leaves, leading to enhanced tea leaf coloration. However, the underlying regulatory mechanism remains unclear. In this study, we revealed that the expressions of chlorophyll synthesis genes were significantly induced by shading, specially, the gene encoding protochlorophyllide oxidoreductase (CsPOR). Indoor control experiment showed that decreased light intensity could significantly induce the expression of CsPOR, and thus cause the increase of chlorophyll content. Subsequently, we explored the light signaling pathway transcription factors regulating chlorophyll synthesis, including CsPIFs and CsHY5. Through expression level and subcellular localization analysis, we found that CsPIF3-2, CsPIF7-1, and CsHY5 may be candidate transcriptional regulators. Transcriptional activation experiments proved that CsHY5 inhibits CsPORL-2 transcription. In summary, we concluded that shading might promote the expression of CsPORL-2 by inhibiting the expression of CsHY5, leading to high accumulation of chlorophyll in tea leaves. The results of this study provide insights into the mechanism regulating the improvements to tea plant quality caused by shading.
format	article
author	Jiaming Chen Jiaming Chen Shuhua Wu Shuhua Wu Fang Dong Jianlong Li Lanting Zeng Jinchi Tang Dachuan Gu
author_facet	Jiaming Chen Jiaming Chen Shuhua Wu Shuhua Wu Fang Dong Jianlong Li Lanting Zeng Jinchi Tang Dachuan Gu
author_sort	Jiaming Chen
title	Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
title_short	Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
title_full	Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
title_fullStr	Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
title_full_unstemmed	Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves
title_sort	mechanism underlying the shading-induced chlorophyll accumulation in tea leaves
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6fecfc118a8d448697e6b91e04948f8f
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Mechanism Underlying the Shading-Induced Chlorophyll Accumulation in Tea Leaves

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