miR-519d downregulates LEP expression to inhibit preeclampsia development

The purpose of the current study was to characterize role of microRNA (miR)-519d in trophoblast cells and preeclampsia (PE) development and its potential underlying mechanism. Regulation of leptin (LEP) by miR-519d was verified using a dual-luciferase reporter gene assay. Loss- and gain-of-function...

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Autores principales:	Cai Hairui, Li Dongmei, Wu Jun, Shi Chunbo
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	microrna-519d leptin preeclampsia trophoblast cells Medicine R
Acceso en línea:	https://doaj.org/article/7043e52e408e467abea727c57fb9262f
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spelling	oai:doaj.org-article:7043e52e408e467abea727c57fb9262f2021-12-05T14:10:54ZmiR-519d downregulates LEP expression to inhibit preeclampsia development2391-546310.1515/med-2021-0244https://doaj.org/article/7043e52e408e467abea727c57fb9262f2021-08-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0244https://doaj.org/toc/2391-5463The purpose of the current study was to characterize role of microRNA (miR)-519d in trophoblast cells and preeclampsia (PE) development and its potential underlying mechanism. Regulation of leptin (LEP) by miR-519d was verified using a dual-luciferase reporter gene assay. Loss- and gain-of-function assays were conducted to detect the roles of miR-519d and LEP in proliferation, migratory ability, and invasive capacity of HTR-8/SVneo cells by means of CCK-8 assay, scratch test, and Transwell invasion assay, respectively. The cell apoptosis rate and cycle distribution were analyzed by flow cytometry. LEP expression was elevated, whereas miR-519d level was suppressed in the PE placenta samples compared with those from normal pregnancy. Depletion of LEP promoted proliferation, migratory ability, and invasive capacity and repressed apoptosis. miR-519d could bind 3′ untranslated regions (3′UTRs) of LEP, the extent of which correlated negatively with LEP expression. miR-519d suppressed the expression of LEP in HTR-8/SVneo cells. Moreover, overexpression of miR-519d promoted survival and migratory ability of HTR-8/SVneo cells. Taken together, we find that miR-519d targeted LEP and downregulated its expression, which could likely inhibit the development of PE.Cai HairuiLi DongmeiWu JunShi ChunboDe Gruyterarticlemicrorna-519dleptinpreeclampsiatrophoblast cellsMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1215-1227 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna-519d leptin preeclampsia trophoblast cells Medicine R
spellingShingle	microrna-519d leptin preeclampsia trophoblast cells Medicine R Cai Hairui Li Dongmei Wu Jun Shi Chunbo miR-519d downregulates LEP expression to inhibit preeclampsia development
description	The purpose of the current study was to characterize role of microRNA (miR)-519d in trophoblast cells and preeclampsia (PE) development and its potential underlying mechanism. Regulation of leptin (LEP) by miR-519d was verified using a dual-luciferase reporter gene assay. Loss- and gain-of-function assays were conducted to detect the roles of miR-519d and LEP in proliferation, migratory ability, and invasive capacity of HTR-8/SVneo cells by means of CCK-8 assay, scratch test, and Transwell invasion assay, respectively. The cell apoptosis rate and cycle distribution were analyzed by flow cytometry. LEP expression was elevated, whereas miR-519d level was suppressed in the PE placenta samples compared with those from normal pregnancy. Depletion of LEP promoted proliferation, migratory ability, and invasive capacity and repressed apoptosis. miR-519d could bind 3′ untranslated regions (3′UTRs) of LEP, the extent of which correlated negatively with LEP expression. miR-519d suppressed the expression of LEP in HTR-8/SVneo cells. Moreover, overexpression of miR-519d promoted survival and migratory ability of HTR-8/SVneo cells. Taken together, we find that miR-519d targeted LEP and downregulated its expression, which could likely inhibit the development of PE.
format	article
author	Cai Hairui Li Dongmei Wu Jun Shi Chunbo
author_facet	Cai Hairui Li Dongmei Wu Jun Shi Chunbo
author_sort	Cai Hairui
title	miR-519d downregulates LEP expression to inhibit preeclampsia development
title_short	miR-519d downregulates LEP expression to inhibit preeclampsia development
title_full	miR-519d downregulates LEP expression to inhibit preeclampsia development
title_fullStr	miR-519d downregulates LEP expression to inhibit preeclampsia development
title_full_unstemmed	miR-519d downregulates LEP expression to inhibit preeclampsia development
title_sort	mir-519d downregulates lep expression to inhibit preeclampsia development
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/7043e52e408e467abea727c57fb9262f
work_keys_str_mv	AT caihairui mir519ddownregulateslepexpressiontoinhibitpreeclampsiadevelopment AT lidongmei mir519ddownregulateslepexpressiontoinhibitpreeclampsiadevelopment AT wujun mir519ddownregulateslepexpressiontoinhibitpreeclampsiadevelopment AT shichunbo mir519ddownregulateslepexpressiontoinhibitpreeclampsiadevelopment
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miR-519d downregulates LEP expression to inhibit preeclampsia development

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