Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry

Data-independent acquisition (DIA)-based proteomics often relies on mass spectrum libraries from data-dependent acquisition experiments. Here, the authors present a method to generate DIA-based chromatogram libraries, enabling DIA-only workflows and detecting more peptides than with spectrum librari...

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Main Authors:	Brian C. Searle, Lindsay K. Pino, Jarrett D. Egertson, Ying S. Ting, Robert T. Lawrence, Brendan X. MacLean, Judit Villén, Michael J. MacCoss
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/71667bdd5c6f47bf95aa8da07df2ca8c
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id	oai:doaj.org-article:71667bdd5c6f47bf95aa8da07df2ca8c
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spelling	oai:doaj.org-article:71667bdd5c6f47bf95aa8da07df2ca8c2021-12-02T16:56:38ZChromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry10.1038/s41467-018-07454-w2041-1723https://doaj.org/article/71667bdd5c6f47bf95aa8da07df2ca8c2018-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07454-whttps://doaj.org/toc/2041-1723Data-independent acquisition (DIA)-based proteomics often relies on mass spectrum libraries from data-dependent acquisition experiments. Here, the authors present a method to generate DIA-based chromatogram libraries, enabling DIA-only workflows and detecting more peptides than with spectrum libraries alone.Brian C. SearleLindsay K. PinoJarrett D. EgertsonYing S. TingRobert T. LawrenceBrendan X. MacLeanJudit VillénMichael J. MacCossNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Brian C. Searle Lindsay K. Pino Jarrett D. Egertson Ying S. Ting Robert T. Lawrence Brendan X. MacLean Judit Villén Michael J. MacCoss Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
description	Data-independent acquisition (DIA)-based proteomics often relies on mass spectrum libraries from data-dependent acquisition experiments. Here, the authors present a method to generate DIA-based chromatogram libraries, enabling DIA-only workflows and detecting more peptides than with spectrum libraries alone.
format	article
author	Brian C. Searle Lindsay K. Pino Jarrett D. Egertson Ying S. Ting Robert T. Lawrence Brendan X. MacLean Judit Villén Michael J. MacCoss
author_facet	Brian C. Searle Lindsay K. Pino Jarrett D. Egertson Ying S. Ting Robert T. Lawrence Brendan X. MacLean Judit Villén Michael J. MacCoss
author_sort	Brian C. Searle
title	Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
title_short	Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
title_full	Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
title_fullStr	Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
title_full_unstemmed	Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
title_sort	chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/71667bdd5c6f47bf95aa8da07df2ca8c
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Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry

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