Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages

Human immunodeficiency virus type 1 (HIV-1) modulates the host cell cycle. The HIV-1 accessory protein Vpr arrests the cell cycle at the G2 phase in dividing cells, and the ability of Vpr to induce G2 arrest is well conserved among primate lentiviruses. Additionally, Vpr-mediated G2 arrest likely co...

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Autores principales:	Tomoyuki Murakami, Ryosuke Matsuura, Nopporn Chutiwitoonchai, Masami Takei, Yoko Aida
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HIV-1 Vpr G2 arrest HIP1 macrophage Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/718ccc91f4b649698b4b464a00452c01
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spelling	oai:doaj.org-article:718ccc91f4b649698b4b464a00452c012021-11-25T19:14:26ZHuntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages10.3390/v131123081999-4915https://doaj.org/article/718ccc91f4b649698b4b464a00452c012021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2308https://doaj.org/toc/1999-4915Human immunodeficiency virus type 1 (HIV-1) modulates the host cell cycle. The HIV-1 accessory protein Vpr arrests the cell cycle at the G2 phase in dividing cells, and the ability of Vpr to induce G2 arrest is well conserved among primate lentiviruses. Additionally, Vpr-mediated G2 arrest likely correlates with enhanced HIV-1 infection in monocyte-derived macrophages. Here, we screened small-interfering RNA to reveal candidates that suppress Vpr-induced G2 arrest and identified Huntingtin-interacting protein 1 (HIP1) required for efficient G2 arrest. Interestingly, HIP1 was not essential for Vpr-induced DNA double-strand breaks, which are required for activation of the DNA-damage checkpoint and G2 arrest. Furthermore, HIP1 knockdown suppressed HIV-1 infection in monocyte-derived macrophages. This study identifies HIP1 as a factor promoting Vpr-induced G2 arrest and HIV-1 infection in macrophages.Tomoyuki MurakamiRyosuke MatsuuraNopporn ChutiwitoonchaiMasami TakeiYoko AidaMDPI AGarticleHIV-1VprG2 arrestHIP1macrophageMicrobiologyQR1-502ENViruses, Vol 13, Iss 2308, p 2308 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV-1 Vpr G2 arrest HIP1 macrophage Microbiology QR1-502
spellingShingle	HIV-1 Vpr G2 arrest HIP1 macrophage Microbiology QR1-502 Tomoyuki Murakami Ryosuke Matsuura Nopporn Chutiwitoonchai Masami Takei Yoko Aida Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
description	Human immunodeficiency virus type 1 (HIV-1) modulates the host cell cycle. The HIV-1 accessory protein Vpr arrests the cell cycle at the G2 phase in dividing cells, and the ability of Vpr to induce G2 arrest is well conserved among primate lentiviruses. Additionally, Vpr-mediated G2 arrest likely correlates with enhanced HIV-1 infection in monocyte-derived macrophages. Here, we screened small-interfering RNA to reveal candidates that suppress Vpr-induced G2 arrest and identified Huntingtin-interacting protein 1 (HIP1) required for efficient G2 arrest. Interestingly, HIP1 was not essential for Vpr-induced DNA double-strand breaks, which are required for activation of the DNA-damage checkpoint and G2 arrest. Furthermore, HIP1 knockdown suppressed HIV-1 infection in monocyte-derived macrophages. This study identifies HIP1 as a factor promoting Vpr-induced G2 arrest and HIV-1 infection in macrophages.
format	article
author	Tomoyuki Murakami Ryosuke Matsuura Nopporn Chutiwitoonchai Masami Takei Yoko Aida
author_facet	Tomoyuki Murakami Ryosuke Matsuura Nopporn Chutiwitoonchai Masami Takei Yoko Aida
author_sort	Tomoyuki Murakami
title	Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
title_short	Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
title_full	Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
title_fullStr	Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
title_full_unstemmed	Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages
title_sort	huntingtin-interacting protein 1 promotes vpr-induced g2 arrest and hiv-1 infection in macrophages
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/718ccc91f4b649698b4b464a00452c01
work_keys_str_mv	AT tomoyukimurakami huntingtininteractingprotein1promotesvprinducedg2arrestandhiv1infectioninmacrophages AT ryosukematsuura huntingtininteractingprotein1promotesvprinducedg2arrestandhiv1infectioninmacrophages AT noppornchutiwitoonchai huntingtininteractingprotein1promotesvprinducedg2arrestandhiv1infectioninmacrophages AT masamitakei huntingtininteractingprotein1promotesvprinducedg2arrestandhiv1infectioninmacrophages AT yokoaida huntingtininteractingprotein1promotesvprinducedg2arrestandhiv1infectioninmacrophages
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Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages

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