Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes.
The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture...
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2013
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oai:doaj.org-article:72352486e23d439e8ed96457071ba73e2021-11-18T08:44:43ZGene expression signature of normal cell-of-origin predicts ovarian tumor outcomes.1932-620310.1371/journal.pone.0080314https://doaj.org/article/72352486e23d439e8ed96457071ba73e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24303006/?tool=EBIhttps://doaj.org/toc/1932-6203The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture of paired normal human ovarian (OV) and fallopian tube (FT) epithelial cells from donors without cancer. While these cells have been cultured individually for short periods of time, to our knowledge this is the first long-term culture of both cell types from the same donors. Through analysis of the gene expression profiles of the cultured OV/FT cells we identified a normal cell-of-origin gene signature that classified primary ovarian cancers into OV-like and FT-like subgroups; this classification correlated with significant differences in clinical outcomes. The identification of a prognostically significant gene expression signature derived solely from normal untransformed cells is consistent with the hypothesis that the normal cell-of-origin may be a source of ovarian tumor heterogeneity and the associated differences in tumor outcome.Melissa A MerrittStefan BentinkMatthew SchwedeMarcin P IwanickiJohn QuackenbushTerri WooElin S AgostonFerenc ReinhardtChristopher P CrumRoss S BerkowitzSamuel C MokAbigail E WittMichelle A JonesBin WangTan A IncePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80314 (2013) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Melissa A Merritt Stefan Bentink Matthew Schwede Marcin P Iwanicki John Quackenbush Terri Woo Elin S Agoston Ferenc Reinhardt Christopher P Crum Ross S Berkowitz Samuel C Mok Abigail E Witt Michelle A Jones Bin Wang Tan A Ince Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| description |
The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture of paired normal human ovarian (OV) and fallopian tube (FT) epithelial cells from donors without cancer. While these cells have been cultured individually for short periods of time, to our knowledge this is the first long-term culture of both cell types from the same donors. Through analysis of the gene expression profiles of the cultured OV/FT cells we identified a normal cell-of-origin gene signature that classified primary ovarian cancers into OV-like and FT-like subgroups; this classification correlated with significant differences in clinical outcomes. The identification of a prognostically significant gene expression signature derived solely from normal untransformed cells is consistent with the hypothesis that the normal cell-of-origin may be a source of ovarian tumor heterogeneity and the associated differences in tumor outcome. |
| format |
article |
| author |
Melissa A Merritt Stefan Bentink Matthew Schwede Marcin P Iwanicki John Quackenbush Terri Woo Elin S Agoston Ferenc Reinhardt Christopher P Crum Ross S Berkowitz Samuel C Mok Abigail E Witt Michelle A Jones Bin Wang Tan A Ince |
| author_facet |
Melissa A Merritt Stefan Bentink Matthew Schwede Marcin P Iwanicki John Quackenbush Terri Woo Elin S Agoston Ferenc Reinhardt Christopher P Crum Ross S Berkowitz Samuel C Mok Abigail E Witt Michelle A Jones Bin Wang Tan A Ince |
| author_sort |
Melissa A Merritt |
| title |
Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| title_short |
Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| title_full |
Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| title_fullStr |
Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| title_full_unstemmed |
Gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| title_sort |
gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/72352486e23d439e8ed96457071ba73e |
| work_keys_str_mv |
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| _version_ |
1718421389518569472 |