Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

Abstract Objective The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not act...

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Autores principales:	Vanegas David, Abril-Novillo Andrea, Khachatryan Aleksandr, Jerves-Andrade Lourdes, Peñaherrera Eugenia, Cuzco Nancy, Wilches Isabel, Calle Jessica, Fabián León-Tamariz
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Essential oils Escherichia coli Microdilution assay Validation Sensitivity Specificity Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/72db53d85e8d4d5bbf99034a349c9fb5
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spelling	oai:doaj.org-article:72db53d85e8d4d5bbf99034a349c9fb52021-12-05T12:18:38ZValidation of a method of broth microdilution for the determination of antibacterial activity of essential oils10.1186/s13104-021-05838-81756-0500https://doaj.org/article/72db53d85e8d4d5bbf99034a349c9fb52021-12-01T00:00:00Zhttps://doi.org/10.1186/s13104-021-05838-8https://doaj.org/toc/1756-0500Abstract Objective The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v). Results A stable emulsion formation was achieved with the addition of Tween 80 and constant agitation, guaranteeing the continuous contact of oil with bacteria (critical step in the microdilution method). The statistical analysis of results obtained with both methods presented a good sensitivity and specificity (100% in both cases), which let us correctly discriminate between active and non-active oils. The values obtained for the minimal inhibitory concentration were independent of the technique used. Finally, the obtained results show that the validated microtechnique allows important diminishment of time and resources for investigations dealing with essential oils or lipophilic extracts evaluation.Vanegas DavidAbril-Novillo AndreaKhachatryan AleksandrJerves-Andrade LourdesPeñaherrera EugeniaCuzco NancyWilches IsabelCalle JessicaFabián León-TamarizBMCarticleEssential oilsEscherichia coliMicrodilution assayValidationSensitivitySpecificityMedicineRBiology (General)QH301-705.5Science (General)Q1-390ENBMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Essential oils Escherichia coli Microdilution assay Validation Sensitivity Specificity Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
spellingShingle	Essential oils Escherichia coli Microdilution assay Validation Sensitivity Specificity Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Vanegas David Abril-Novillo Andrea Khachatryan Aleksandr Jerves-Andrade Lourdes Peñaherrera Eugenia Cuzco Nancy Wilches Isabel Calle Jessica Fabián León-Tamariz Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
description	Abstract Objective The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v). Results A stable emulsion formation was achieved with the addition of Tween 80 and constant agitation, guaranteeing the continuous contact of oil with bacteria (critical step in the microdilution method). The statistical analysis of results obtained with both methods presented a good sensitivity and specificity (100% in both cases), which let us correctly discriminate between active and non-active oils. The values obtained for the minimal inhibitory concentration were independent of the technique used. Finally, the obtained results show that the validated microtechnique allows important diminishment of time and resources for investigations dealing with essential oils or lipophilic extracts evaluation.
format	article
author	Vanegas David Abril-Novillo Andrea Khachatryan Aleksandr Jerves-Andrade Lourdes Peñaherrera Eugenia Cuzco Nancy Wilches Isabel Calle Jessica Fabián León-Tamariz
author_facet	Vanegas David Abril-Novillo Andrea Khachatryan Aleksandr Jerves-Andrade Lourdes Peñaherrera Eugenia Cuzco Nancy Wilches Isabel Calle Jessica Fabián León-Tamariz
author_sort	Vanegas David
title	Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
title_short	Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
title_full	Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
title_fullStr	Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
title_full_unstemmed	Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
title_sort	validation of a method of broth microdilution for the determination of antibacterial activity of essential oils
publisher	BMC
publishDate	2021
url	https://doaj.org/article/72db53d85e8d4d5bbf99034a349c9fb5
work_keys_str_mv	AT vanegasdavid validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT abrilnovilloandrea validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT khachatryanaleksandr validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT jervesandradelourdes validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT penaherreraeugenia validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT cuzconancy validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT wilchesisabel validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT callejessica validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils AT fabianleontamariz validationofamethodofbrothmicrodilutionforthedeterminationofantibacterialactivityofessentialoils
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Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

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