High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

In vitro chemotactic assays are often 2D, low-throughput and lack fine gradient control. Here the authors present a hydrogel-based microfluidic platform for high-throughput 3D chemotactic assays, and use it to study neuronal sensitivity to guidance molecule gradient steepness.

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Autores principales:	Zhen Xu, Peilin Fang, Bingzhe Xu, Yufeng Lu, Jinghui Xiong, Feng Gao, Xin Wang, Jun Fan, Peng Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7319b5646b654ef192654ef80c5613a9
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spelling	oai:doaj.org-article:7319b5646b654ef192654ef80c5613a92021-12-02T14:41:16ZHigh-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients10.1038/s41467-018-07186-x2041-1723https://doaj.org/article/7319b5646b654ef192654ef80c5613a92018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07186-xhttps://doaj.org/toc/2041-1723In vitro chemotactic assays are often 2D, low-throughput and lack fine gradient control. Here the authors present a hydrogel-based microfluidic platform for high-throughput 3D chemotactic assays, and use it to study neuronal sensitivity to guidance molecule gradient steepness.Zhen XuPeilin FangBingzhe XuYufeng LuJinghui XiongFeng GaoXin WangJun FanPeng ShiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Zhen Xu Peilin Fang Bingzhe Xu Yufeng Lu Jinghui Xiong Feng Gao Xin Wang Jun Fan Peng Shi High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
description	In vitro chemotactic assays are often 2D, low-throughput and lack fine gradient control. Here the authors present a hydrogel-based microfluidic platform for high-throughput 3D chemotactic assays, and use it to study neuronal sensitivity to guidance molecule gradient steepness.
format	article
author	Zhen Xu Peilin Fang Bingzhe Xu Yufeng Lu Jinghui Xiong Feng Gao Xin Wang Jun Fan Peng Shi
author_facet	Zhen Xu Peilin Fang Bingzhe Xu Yufeng Lu Jinghui Xiong Feng Gao Xin Wang Jun Fan Peng Shi
author_sort	Zhen Xu
title	High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
title_short	High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
title_full	High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
title_fullStr	High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
title_full_unstemmed	High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
title_sort	high-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7319b5646b654ef192654ef80c5613a9
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High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

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