HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration

Abstract Background We have reported that heparin-binding epidermal growth factor (HB-EGF) is increased in patients with chronic obstructive pulmonary disease (COPD) and associated with collagen deposition, but the mechanisms remain unclear. In the present study, we aimed to investigated the inflamm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanyu Li, Guomei Su, Yu Zhong, Zhilin Xiong, Tong Huang, Jingyun Quan, Jiewen Huang, Xiaoxia Wen, Chaole Luo, Weilin Zheng, Jinfeng Chen, Junfen Cheng, Weimin Yao, Tianwen Lai
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	HB-EGF Lung fibrosis IL-8 Airway epithelium Diseases of the respiratory system RC705-779
Acceso en línea:	https://doaj.org/article/733cb9ea5b4e46acbf785e42bce2d5c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:733cb9ea5b4e46acbf785e42bce2d5c7
record_format	dspace
spelling	oai:doaj.org-article:733cb9ea5b4e46acbf785e42bce2d5c72021-11-08T11:13:25ZHB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration10.1186/s12890-021-01726-w1471-2466https://doaj.org/article/733cb9ea5b4e46acbf785e42bce2d5c72021-11-01T00:00:00Zhttps://doi.org/10.1186/s12890-021-01726-whttps://doaj.org/toc/1471-2466Abstract Background We have reported that heparin-binding epidermal growth factor (HB-EGF) is increased in patients with chronic obstructive pulmonary disease (COPD) and associated with collagen deposition, but the mechanisms remain unclear. In the present study, we aimed to investigated the inflammatory cytokines secreted by bronchial epithelial cells following exposure to HB-EGF that promoted proliferation and migration of human lung fibroblast. Methods HB-EGF–induced inflammatory cytokines were assayed in two airway epithelial cells (primary human bronchial epithelial cells [HBECs] and BEAS-2B cells). Moreover, the culture supernatants derived from HB-EGF-treated HBECs and BEAS-2B cells were added to human primary lung fibroblasts. The effect of culture supernatants on proliferation and migration of fibroblasts was assessed. Results IL-8 expression was significantly increased in bronchial epithelial cells treated with HB-EGF, which was at least partially dependent on NF-kB pathways activation. HB-EGF–induced IL-8 was found to further promote lung fibroblasts proliferation and migration, and the effects were attenuated after neutralizing IL-8. Conclusions These findings suggest that HB-EGF may be involved in the pathology of airway fibrosis by induction of IL-8 from airway epithelium, subsequently causing lung fibroblasts proliferation and migration. Thus, inhibition of HBEGF and/or IL-8 production could prevent the development of airway fibrosis by modulating fibroblast activation.Yanyu LiGuomei SuYu ZhongZhilin XiongTong HuangJingyun QuanJiewen HuangXiaoxia WenChaole LuoWeilin ZhengJinfeng ChenJunfen ChengWeimin YaoTianwen LaiBMCarticleHB-EGFLung fibrosisIL-8Airway epitheliumDiseases of the respiratory systemRC705-779ENBMC Pulmonary Medicine, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HB-EGF Lung fibrosis IL-8 Airway epithelium Diseases of the respiratory system RC705-779
spellingShingle	HB-EGF Lung fibrosis IL-8 Airway epithelium Diseases of the respiratory system RC705-779 Yanyu Li Guomei Su Yu Zhong Zhilin Xiong Tong Huang Jingyun Quan Jiewen Huang Xiaoxia Wen Chaole Luo Weilin Zheng Jinfeng Chen Junfen Cheng Weimin Yao Tianwen Lai HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
description	Abstract Background We have reported that heparin-binding epidermal growth factor (HB-EGF) is increased in patients with chronic obstructive pulmonary disease (COPD) and associated with collagen deposition, but the mechanisms remain unclear. In the present study, we aimed to investigated the inflammatory cytokines secreted by bronchial epithelial cells following exposure to HB-EGF that promoted proliferation and migration of human lung fibroblast. Methods HB-EGF–induced inflammatory cytokines were assayed in two airway epithelial cells (primary human bronchial epithelial cells [HBECs] and BEAS-2B cells). Moreover, the culture supernatants derived from HB-EGF-treated HBECs and BEAS-2B cells were added to human primary lung fibroblasts. The effect of culture supernatants on proliferation and migration of fibroblasts was assessed. Results IL-8 expression was significantly increased in bronchial epithelial cells treated with HB-EGF, which was at least partially dependent on NF-kB pathways activation. HB-EGF–induced IL-8 was found to further promote lung fibroblasts proliferation and migration, and the effects were attenuated after neutralizing IL-8. Conclusions These findings suggest that HB-EGF may be involved in the pathology of airway fibrosis by induction of IL-8 from airway epithelium, subsequently causing lung fibroblasts proliferation and migration. Thus, inhibition of HBEGF and/or IL-8 production could prevent the development of airway fibrosis by modulating fibroblast activation.
format	article
author	Yanyu Li Guomei Su Yu Zhong Zhilin Xiong Tong Huang Jingyun Quan Jiewen Huang Xiaoxia Wen Chaole Luo Weilin Zheng Jinfeng Chen Junfen Cheng Weimin Yao Tianwen Lai
author_facet	Yanyu Li Guomei Su Yu Zhong Zhilin Xiong Tong Huang Jingyun Quan Jiewen Huang Xiaoxia Wen Chaole Luo Weilin Zheng Jinfeng Chen Junfen Cheng Weimin Yao Tianwen Lai
author_sort	Yanyu Li
title	HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
title_short	HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
title_full	HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
title_fullStr	HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
title_full_unstemmed	HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
title_sort	hb-egf-induced il-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration
publisher	BMC
publishDate	2021
url	https://doaj.org/article/733cb9ea5b4e46acbf785e42bce2d5c7
work_keys_str_mv	AT yanyuli hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT guomeisu hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT yuzhong hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT zhilinxiong hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT tonghuang hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT jingyunquan hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT jiewenhuang hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT xiaoxiawen hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT chaoleluo hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT weilinzheng hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT jinfengchen hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT junfencheng hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT weiminyao hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration AT tianwenlai hbegfinducedil8secretionfromairwayepitheliumleadstolungfibroblastproliferationandmigration
_version_	1718442344930344960

HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration

Ejemplares similares