A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs

The objective of this study was to assess whether diets with increased resistant starch (RS) had a positive effect on markers of colonic health in dogs. Three identical diets were extruded with high, medium and low shear (HS, MS and LS) to incrementally increase RS, and fed to 24 dogs in a replicate...

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Autores principales:	Isabella Corsato Alvarenga, Matthew I. Jackson, Dennis E. Jewell, Charles G. Aldrich
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dog microbiota resistant starch extrusion corn metabolomics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/737f684902bc4b10be8515e02f21eb9b
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spelling	oai:doaj.org-article:737f684902bc4b10be8515e02f21eb9b2021-11-25T18:24:54ZA Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs10.3390/microorganisms91122932076-2607https://doaj.org/article/737f684902bc4b10be8515e02f21eb9b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2293https://doaj.org/toc/2076-2607The objective of this study was to assess whether diets with increased resistant starch (RS) had a positive effect on markers of colonic health in dogs. Three identical diets were extruded with high, medium and low shear (HS, MS and LS) to incrementally increase RS, and fed to 24 dogs in a replicated 3 × 3 William’s Latin square design for 28-day periods. Fasting blood and fresh feces were collected on the last week of each period. Fecal quality was maintained among treatments. Gut integrity markers were measured by ELISA. Fecal short-chain fatty acids (SCFAs) were measured by LC MS/MS. In addition, the microbiota of dogs was determined from fresh feces by 16s rRNA high throughput sequencing. Untargeted metabolomics of both feces and serum were determined by UPLC. Data were analyzed using mixed models. There were no treatment effects on satiety hormones or gut integrity markers. Dogs fed LS or MS diets had marginal evidence (<i>p</i> < 0.10) for decreased fecal pH and for higher concentration (<i>p</i> < 0.05) of butyric acid and fecal oligosaccharides, succinate and lactate. Also, dogs fed the MS or LS diets had a shift towards more saccharolytic bacteria.Isabella Corsato AlvarengaMatthew I. JacksonDennis E. JewellCharles G. AldrichMDPI AGarticledogmicrobiotaresistant starchextrusioncornmetabolomicsBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2293, p 2293 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dog microbiota resistant starch extrusion corn metabolomics Biology (General) QH301-705.5
spellingShingle	dog microbiota resistant starch extrusion corn metabolomics Biology (General) QH301-705.5 Isabella Corsato Alvarenga Matthew I. Jackson Dennis E. Jewell Charles G. Aldrich A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
description	The objective of this study was to assess whether diets with increased resistant starch (RS) had a positive effect on markers of colonic health in dogs. Three identical diets were extruded with high, medium and low shear (HS, MS and LS) to incrementally increase RS, and fed to 24 dogs in a replicated 3 × 3 William’s Latin square design for 28-day periods. Fasting blood and fresh feces were collected on the last week of each period. Fecal quality was maintained among treatments. Gut integrity markers were measured by ELISA. Fecal short-chain fatty acids (SCFAs) were measured by LC MS/MS. In addition, the microbiota of dogs was determined from fresh feces by 16s rRNA high throughput sequencing. Untargeted metabolomics of both feces and serum were determined by UPLC. Data were analyzed using mixed models. There were no treatment effects on satiety hormones or gut integrity markers. Dogs fed LS or MS diets had marginal evidence (<i>p</i> < 0.10) for decreased fecal pH and for higher concentration (<i>p</i> < 0.05) of butyric acid and fecal oligosaccharides, succinate and lactate. Also, dogs fed the MS or LS diets had a shift towards more saccharolytic bacteria.
format	article
author	Isabella Corsato Alvarenga Matthew I. Jackson Dennis E. Jewell Charles G. Aldrich
author_facet	Isabella Corsato Alvarenga Matthew I. Jackson Dennis E. Jewell Charles G. Aldrich
author_sort	Isabella Corsato Alvarenga
title	A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
title_short	A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
title_full	A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
title_fullStr	A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
title_full_unstemmed	A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs
title_sort	low to medium-shear extruded kibble with greater resistant starch increased fecal oligosaccharides, butyric acid, and other saccharolytic fermentation by-products in dogs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/737f684902bc4b10be8515e02f21eb9b
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A Low to Medium-Shear Extruded Kibble with Greater Resistant Starch Increased Fecal Oligosaccharides, Butyric Acid, and Other Saccharolytic Fermentation By-Products in Dogs

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