Engineering subtilisin proteases that specifically degrade active RAS

Engineering subtilisin proteases that specifically degrade active RAS

Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity. Proteases engineered to cleave this region degraded RAS in vitro...

Full description

Saved in:
Bibliographic Details
Main Authors: Yingwei Chen, Eric A. Toth, Biao Ruan, Eun Jung Choi, Richard Simmerman, Yihong Chen, Yanan He, Ruixue Wang, Raquel Godoy-Ruiz, Harlan King, Gregory Custer, D. Travis Gallagher, David A. Rozak, Melani Solomon, Silvia Muro, David J. Weber, John Orban, Thomas R. Fuerst, Philip N. Bryan
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Biology (General)
QH301-705.5
Online Access:https://doaj.org/article/742c1a3e0584400b8b631c6e4a1b0ff6
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doaj.org/article/742c1a3e0584400b8b631c6e4a1b0ff6

Similar Items