Amplification-free RNA detection with CRISPR–Cas13

The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a proof-of-concept, they demonstrate its utility in detecting the SARS-CoV-2 N gene at a minimum concentration of ~5 fM, which is m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hajime Shinoda, Yuya Taguchi, Ryoya Nakagawa, Asami Makino, Sae Okazaki, Masahiro Nakano, Yukiko Muramoto, Chiharu Takahashi, Ikuko Takahashi, Jun Ando, Takeshi Noda, Osamu Nureki, Hiroshi Nishimasu, Rikiya Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/74b9926452694c26addf7fb0b414e93e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:74b9926452694c26addf7fb0b414e93e
record_format	dspace
spelling	oai:doaj.org-article:74b9926452694c26addf7fb0b414e93e2021-12-02T18:28:59ZAmplification-free RNA detection with CRISPR–Cas1310.1038/s42003-021-02001-82399-3642https://doaj.org/article/74b9926452694c26addf7fb0b414e93e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02001-8https://doaj.org/toc/2399-3642The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a proof-of-concept, they demonstrate its utility in detecting the SARS-CoV-2 N gene at a minimum concentration of ~5 fM, which is much lower than other amplification-free CRISPR-Cas-based methods.Hajime ShinodaYuya TaguchiRyoya NakagawaAsami MakinoSae OkazakiMasahiro NakanoYukiko MuramotoChiharu TakahashiIkuko TakahashiJun AndoTakeshi NodaOsamu NurekiHiroshi NishimasuRikiya WatanabeNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Hajime Shinoda Yuya Taguchi Ryoya Nakagawa Asami Makino Sae Okazaki Masahiro Nakano Yukiko Muramoto Chiharu Takahashi Ikuko Takahashi Jun Ando Takeshi Noda Osamu Nureki Hiroshi Nishimasu Rikiya Watanabe Amplification-free RNA detection with CRISPR–Cas13
description	The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a proof-of-concept, they demonstrate its utility in detecting the SARS-CoV-2 N gene at a minimum concentration of ~5 fM, which is much lower than other amplification-free CRISPR-Cas-based methods.
format	article
author	Hajime Shinoda Yuya Taguchi Ryoya Nakagawa Asami Makino Sae Okazaki Masahiro Nakano Yukiko Muramoto Chiharu Takahashi Ikuko Takahashi Jun Ando Takeshi Noda Osamu Nureki Hiroshi Nishimasu Rikiya Watanabe
author_facet	Hajime Shinoda Yuya Taguchi Ryoya Nakagawa Asami Makino Sae Okazaki Masahiro Nakano Yukiko Muramoto Chiharu Takahashi Ikuko Takahashi Jun Ando Takeshi Noda Osamu Nureki Hiroshi Nishimasu Rikiya Watanabe
author_sort	Hajime Shinoda
title	Amplification-free RNA detection with CRISPR–Cas13
title_short	Amplification-free RNA detection with CRISPR–Cas13
title_full	Amplification-free RNA detection with CRISPR–Cas13
title_fullStr	Amplification-free RNA detection with CRISPR–Cas13
title_full_unstemmed	Amplification-free RNA detection with CRISPR–Cas13
title_sort	amplification-free rna detection with crispr–cas13
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/74b9926452694c26addf7fb0b414e93e
work_keys_str_mv	AT hajimeshinoda amplificationfreernadetectionwithcrisprcas13 AT yuyataguchi amplificationfreernadetectionwithcrisprcas13 AT ryoyanakagawa amplificationfreernadetectionwithcrisprcas13 AT asamimakino amplificationfreernadetectionwithcrisprcas13 AT saeokazaki amplificationfreernadetectionwithcrisprcas13 AT masahironakano amplificationfreernadetectionwithcrisprcas13 AT yukikomuramoto amplificationfreernadetectionwithcrisprcas13 AT chiharutakahashi amplificationfreernadetectionwithcrisprcas13 AT ikukotakahashi amplificationfreernadetectionwithcrisprcas13 AT junando amplificationfreernadetectionwithcrisprcas13 AT takeshinoda amplificationfreernadetectionwithcrisprcas13 AT osamunureki amplificationfreernadetectionwithcrisprcas13 AT hiroshinishimasu amplificationfreernadetectionwithcrisprcas13 AT rikiyawatanabe amplificationfreernadetectionwithcrisprcas13
_version_	1718377985481900032

Amplification-free RNA detection with CRISPR–Cas13

Ejemplares similares