Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.
Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expressi...
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Main Authors: | , , , |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2012
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Subjects: | |
Online Access: | https://doaj.org/article/7554dccdbaca46a4b17e496fdc5d1680 |
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