Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.

Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expressi...

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Bibliographic Details
Main Authors: Kavleen Sikand, Jagjit Singh, Jey Sabith Ebron, Girish C Shukla
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2012
Subjects:
R
Q
Online Access:https://doaj.org/article/7554dccdbaca46a4b17e496fdc5d1680
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